Dose-Dependent Shift in Relative Contribution of Homologous Recombination to DNA Repair after Low-LET Ionizing Radiation Exposure: Empirical Evidence and Numerical Simulation

被引：3

作者：

Belov, Oleg ^{[1
,2
,3
]}

Chigasova, Anna ^{[4
,5
]}

Pustovalova, Margarita ^{[6
,7
]}

Osipov, Andrey ^{[4
]}

Eremin, Petr ^{[8
]}

Vorobyeva, Natalia ^{[4
,6
]}

Osipov, Andreyan N. ^{[1
,4
,6
,7
]}

机构：

[1] Joint Inst Nucl Res, 6 Joliot Curie St, Dubna 141980, Russia

[2] Russian Acad Sci, Inst Biomed Problems, 76A Khoroshevskoye Shosse, Moscow 123007, Russia

[3] Dubna State Univ, Inst Syst Anal & Management, 19 Univ Skaya St, Dubna 141980, Russia

[4] Russian Acad Sci, NN Semenov Fed Res Ctr Chem Phys, Moscow 119991, Russia

[5] Russian Acad Sci, Emanuel Inst Biochem Phys, Moscow 119334, Russia

[6] Fed Med Biol Agcy SRC FMBC, State Res Ctr, Burnasyan Fed Med Biophys Ctr, Moscow 123098, Russia

[7] Moscow Inst Phys & Technol, Sch Biol & Med Phys, Dolgoprudnyi 141700, Russia

[8] Minist Hlth Russia, FSBI Natl Med Res Ctr Rehabil & Balneol, Moscow 121099, Russia

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2023年 / 45卷 / 09期

基金：

俄罗斯科学基金会;

关键词：

DNA double-strand breaks; ionizing radiation; DNA repair pathways; homologous recombination; mathematical modeling; DOUBLE-STRAND BREAKS; LINEAR-ENERGY-TRANSFER; MAMMALIAN-CELLS; DSB-REPAIR; ADAPTIVE RESPONSE; GAMMA-H2AX FOCI; DAMAGE; MODEL; KINETICS; STRESS;

D O I：

10.3390/cimb45090465

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding the relative contributions of different repair pathways to radiation-induced DNA damage responses remains a challenging issue in terms of studying the radiation injury endpoints. The comparative manifestation of homologous recombination (HR) after irradiation with different doses greatly determines the overall effectiveness of recovery in a dividing cell after irradiation, since HR is an error-free mechanism intended to perform the repair of DNA double-strand breaks (DSB) during S/G2 phases of the cell cycle. In this article, we present experimentally observed evidence of dose-dependent shifts in the relative contributions of HR in human fibroblasts after X-ray exposure at doses in the range 20-1000 mGy, which is also supported by quantitative modeling of DNA DSB repair. Our findings indicate that the increase in the radiation dose leads to a dose-dependent decrease in the relative contribution of HR in the entire repair process.

引用

页码：7352 / 7373

页数：22

共 3 条

[1] Participation of DNA-PKcs in DSB Repair after Exposure to High- and Low-LET Radiation
Anderson, Jennifer A.
Harper, Jane V.
Cucinotta, Francis A.
O'Neill, Peter
RADIATION RESEARCH, 2010, 174 (02) : 195 - 205
[2] Evaluation of the Contribution of Homologous Recombination in DNA Double-Strand Break Repair in Human Fibroblasts after Exposure to Low and Intermediate Doses of X-ray Radiation
Grekhova, A. K.
Pustovalova, M. V.
Eremin, P. S.
Ozerov, I. V.
Maksimova, O. A.
Gordeev, A. V.
Vorobyeva, N. Yu.
Osipov, A. N.
BIOLOGY BULLETIN, 2019, 46 (11) : 1496 - 1502
[3] Evaluation of the Contribution of Homologous Recombination in DNA Double-Strand Break Repair in Human Fibroblasts after Exposure to Low and Intermediate Doses of X-ray Radiation
A. K. Grekhova
M. V. Pustovalova
P. S. Eremin
I. V. Ozerov
O. A. Maksimova
A. V. Gordeev
N. Yu. Vorobyeva
A. N. Osipov
Biology Bulletin, 2019, 46 : 1496 - 1502

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