Identification, Typing and Drug Resistance of Cronobacter spp. in Powdered Infant Formula and Processing Environment

被引：13

作者：

Li, Hongxuan ^{[1
]}

Fu, Shiqian ^{[2
]}

Song, Danliangmin ^{[1
]}

Qin, Xue ^{[1
]}

Zhang, Wei ^{[1
]}

Man, Chaoxin ^{[1
]}

Yang, Xinyan ^{[1
]}

Jiang, Yujun ^{[1
]}

机构：

[1] Northeast Agr Univ, Dept Food Sci, Key Lab Dairy Sci, Minist Educ, Harbin 150030, Peoples R China

[2] Ningbo Univ, Coll Food & Pharmaceut Sci, Zhejiang Malaysia Joint Res Lab Agr Prod Proc & Nu, Key Lab Anim Prot Food Proc Technol Zhejiang Prov, Ningbo 315800, Zhejiang, Peoples R China

来源：

FOODS | 2023年 / 12卷 / 05期

基金：

黑龙江省自然科学基金;

关键词：

powdered infant formula; Cronobacter spp; antibiotic resistance; multilocus sequence typing; transcriptomics; ENTEROBACTER-SAKAZAKII; VISUAL DETECTION; AMPLIFICATION; DIVERSITY; GENUS;

D O I：

10.3390/foods12051084

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Cronobacter spp. is a food-borne pathogenic microorganism that can cause serious diseases such as meningitis, sepsis, and necrotizing colitis in infants and young children. Powdered infant formula (PIF) is one of the main contamination routes, in which the processing environment is an important source of pollution. In this investigation, 35 Cronobacter strains isolated from PIF and its processing environment were identified and typed by 16S rRNA sequencing and multilocus sequence typing (MLST) technology. A total of 35 sequence types were obtained, and three new sequence types were isolated for the first time. The antibiotic resistance was analyzed, showing that all isolates were resistant to erythromycin but sensitive to ciprofloxacin. Multi-drug resistant strains accounted for 68.57% of the total, among which Cronobacter strains with the strongest drug resistance reached 13 multiple drug resistance. Combined with transcriptomics, 77 differentially expressed genes related to drug resistance were identified. The metabolic pathways were deeply excavated, and under the stimulation of antibiotic conditions, Cronobacter strains can activate the multidrug efflux system by regulating the expression of chemotaxis-related genes, thus, secreting more drug efflux proteins to enhance drug resistance. The study of drug resistance of Cronobacter and its mechanism has important public health significance for the rational selection of existing antibacterial drugs, the development of new antibacterial drugs to reduce the occurrence of bacterial resistance, and the control and treatment of infections caused by Cronobacter.

引用

页数：16