Alpha-ketoglutarate up-regulates autophagic activity in peri-implant environment and enhances dental implant osseointegration in osteoporotic mice

被引：11

作者：

Liu, Ruojing ^{[1
,2
]}

Gao, Yi ^{[3
]}

Huang, Li ^{[1
,4
]}

Shi, Bing ^{[1
,5
]}

Yin, Xing ^{[1
,2
,6
]}

Zou, Shujuan ^{[1
,2
,6
]}

机构：

[1] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China

[2] Sichuan Univ, West China Hosp Stomatol, Dept Orthodont, Chengdu, Peoples R China

[3] Sichuan Univ, West China Hosp Stomatol, Dept Jinjiang Clin, Chengdu, Peoples R China

[4] Hosp Chengdu Univ Tradit Chinese Med, Dept stomatol, Chengdu, Peoples R China

[5] Sichuan Univ, West China Hosp Stomatol, Dept Oral & Maxillofacial Surg, Chengdu, Peoples R China

[6] Sichuan Univ, West China Hosp Stomatol, Dept Orthodont, 14,3rd Sect,Ren Min South Rd, Chengdu 610041, Sichuan, Peoples R China

来源：

JOURNAL OF CLINICAL PERIODONTOLOGY | 2023年 / 50卷 / 05期

基金：

中国国家自然科学基金;

关键词：

alpha-ketoglutarate; autophagy; dental implant; osseointegration; osteogenesis; OSTEOBLAST DIFFERENTIATION; MONITORING AUTOPHAGY; MURINE MODEL; BONE-DENSITY; SODIUM-SALT; OSTEOCYTES;

D O I：

10.1111/jcpe.13784

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Aim: The osseointegration of dental implants is impaired in patients with osteoporosis, leading to significantly higher failure rates. This study set out to investigate the potential effects of alpha-ketoglutarate (alpha-KG) on implant osseointegration in an osteoporotic mouse model. Materials and Methods: Female C57BL/6 mice received ovariectomy and bilateral first maxillary molar extraction at the age of 7 weeks. Dental implants were inserted 8 weeks after tooth extraction. In one of the groups, alpha-KG was administered via drinking water throughout the experimental period. Specimens were collected on post-implant days (PIDs) 3, 7, 14, and 21 for micro-CT, histological, and immunohistochemical analyses. At the same time, bone-marrow-derived mesenchymal stem cells (BMMSCs) treated with alpha-KG were interrogated for osteogenic differentiation, autophagic activity, and apoptosis. Results: alpha-KG supplementation in drinking water resulted in enhanced dental implant osseointegration in ovariectomized mice, with up-regulated osteogenic and autophagic activity and down-regulated osteoclast differentiation and cell apoptosis. alpha-KG-treated BMMSCs showed enhanced activity in proliferation, sur-vival, colony formation, and osteogenic differentiation, as well as autophagic activity. Conclusions: Systemic alpha-KG supplementation effectively prevents the failure of den-tal implant osseointegration in mice under an osteoporotic state.

引用

页码：671 / 683

页数：13