In vivo analysis of the stratum corneum of Japanese neonates and infants using confocal Raman spectroscopy: a pilot study

被引:6
作者
Matsumoto, Yukio [1 ]
Mochimaru, Naoko [1 ]
Yasuda, Hazuki [1 ]
Pak, Kyongsun [1 ]
Kobayashi, Tohru [2 ]
Yamamoto-Hanada, Kiwako [3 ]
Ohya, Yukihiro [3 ]
Kiuchi, Megumi [4 ]
Kurokawa, Masashi [5 ]
Yoshida, Kazue [1 ,3 ]
机构
[1] Natl Ctr Child Hlth & Dev, Div Dermatol, 2-10-1 Okura, Tokyo 1578535, Japan
[2] Natl Ctr Child Hlth & Dev, Ctr Clin Res & Dev, Dept Data Management, Div Biostat, Tokyo, Japan
[3] Natl Ctr Child Hlth & Dev, Allergy Ctr, Tokyo, Japan
[4] Pigeon Corp, Dept Corp Commun, Tokyo, Japan
[5] Pigeon Home Prod Corp, Shizuoka, Japan
关键词
ceramide; cholesterol; confocal Raman spectroscopy; infants; natural moisturizing factor; neonates; stratum corneum; SKIN PHYSIOLOGY; ATOPIC-DERMATITIS; DRY;
D O I
10.1111/srt.13276
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
BackgroundPhysiological skin properties of neonates and infants change drastically after birth and are implicated in the onset of atopic dermatitis and other diseases. Studies have measured physiological skin properties in infants; however, how these properties change over time remains unclear. No reports have measured ceramide in the stratum corneum of infants using confocal Raman spectroscopy; hence, we used it to measure the physiological properties of the skin, including ceramide, in infants.Materials and methodsThe water content and other factors in the skin of infants aged 0, 1, and 6 months were measured. All measurements were performed five times indoors at 22 +/- 2 degrees C and 50% +/- 10% relative humidity in the middle of the calf at 4-mu m distances, and their mean was calculated.ResultsThe water content of the area between the skin surface and superficial layers was the lowest in newborns as compared with other ages, and the deeper the skin layer, the higher the water content. The stratum corneum, evaluated using confocal Raman spectroscopy, was the thickest in newborns and gradually thinned with age. Its water content was the lowest in newborns. The levels of natural moisturizing factor, ceramide, and cholesterol were higher in newborns and tended to decrease with age.ConclusionThis report is the first to evaluate ceramide in the stratum corneum of infants using confocal Raman spectroscopy and could help in conducting subsequent longitudinal measurements of physiological skin properties in neonates and infants.
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页数:6
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共 25 条
[1]   The revised EEMCO guidance for the in vivo measurement of water in the skin [J].
Berardesca, Enzo ;
Loden, Marie ;
Serup, Jorgen ;
Masson, Philippe ;
Rodrigues, Luis Monteiro .
SKIN RESEARCH AND TECHNOLOGY, 2018, 24 (03) :351-358
[2]  
Bielfeldt S, 2009, IFSCC MAGAZINE, V12, P9
[3]   A randomized clinical study on the effects of emollient use on the developing infant skin microbiome and metabolome [J].
Capone, Kimberly A. ;
Friscia, Diana L. ;
Nikolovski, Janeta ;
Telofski, Lorena S. ;
Stamatas, Georgios N. .
EXPERIMENTAL DERMATOLOGY, 2023, 32 (01) :75-77
[4]   In vivo confocal Raman microspectroscopy of the skin:: Noninvasive determination of molecular concentration profiles [J].
Caspers, PJ ;
Lucassen, GW ;
Carter, EA ;
Bruining, HA ;
Puppels, GJ .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 116 (03) :434-442
[5]   Infant epidermal skin physiology: adaptation after birth [J].
Fluhr, J. W. ;
Darlenski, R. ;
Lachmann, N. ;
Baudouin, C. ;
Msika, P. ;
De Belilovsky, C. ;
Hachem, J. -P. .
BRITISH JOURNAL OF DERMATOLOGY, 2012, 166 (03) :483-490
[6]   Age-Dependent Transformation of Skin Biomechanical Properties and Micromorphology during Infancy and Childhood [J].
Fluhr, Joachim W. ;
Bellemere, Gaelle ;
Ferrari, Chiara ;
De Belilovsky, Clarence ;
Boyer, Gaetan ;
Lachmann, Nadege ;
McGuckin, Colin P. ;
Forraz, Nico ;
Darlenski, Razvigor ;
Chadoutaud, Bernard ;
Msika, Philippe ;
Baudouin, Caroline ;
Pellacani, Giovanni .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2019, 139 (02) :464-466
[7]   Applications of Raman spectroscopy in skin research - From skin physiology and diagnosis up to risk assessment and dermal drug delivery [J].
Franzen, Lutz ;
Windbergs, Maike .
ADVANCED DRUG DELIVERY REVIEWS, 2015, 89 :91-104
[8]  
Hoath S. B., 2006, International Journal of Cosmetic Science, V28, P319, DOI 10.1111/j.1467-2494.2006.00338.x
[9]  
Holbrook KA., 1982, NEONATAL SKIN STRUCT, P3
[10]   Transepidermal water loss measurement during infancy can predict the subsequent development of atopic dermatitis regardless of filaggrin mutations [J].
Horimukai, Kenta ;
Morita, Kumiko ;
Narita, Masami ;
Kondo, Mai ;
Kabashima, Shigenori ;
Inoue, Eisuke ;
Sasaki, Takashi ;
Niizeki, Hironori ;
Saito, Hirohisa ;
Matsumoto, Kenji ;
Ohya, Yukihiro .
ALLERGOLOGY INTERNATIONAL, 2016, 65 (01) :103-108