Novel sterol binding domains in bacteria

被引:2
作者
Zhai, Liting [1 ,2 ]
Bonds, Amber C. [3 ]
Smith, Clyde A. [1 ,4 ]
Oo, Hannah [1 ,2 ]
Chou, Jonathan Chiu-Chun [1 ,2 ]
Welander, Paula, V [3 ]
Dassama, Laura M. K. [1 ,2 ,5 ]
机构
[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
[2] Stanford Univ, Sarafan ChEM H, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Earth Syst Sci, Stanford, CA USA
[4] Stanford Univ, Stanford Synchrotron Radiat Lightsource, Stanford, CA USA
[5] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
来源
ELIFE | 2024年 / 12卷
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
bacteria; E; coli; Methylococcus capsulatus; sterols; methanotrophs; TREPONEMA-PALLIDUM; OUTER-MEMBRANE; PROTEIN; CHOLESTEROL; GENE; TRANSPORT; EVOLUTION; MODEL; IDENTIFICATION; DEHYDROGENASE;
D O I
10.7554/eLife.90696
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sterol lipids are widely present in eukaryotes and play essential roles in signaling and modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function in bacteria is not known. Moreover, many more species, including pathogens and commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize sterols, producing a mixture of C-4 methylated sterols that are distinct from those observed in eukaryotes. C-4 methylated sterols are synthesized in the cytosol and localized to the outer membrane, suggesting that a bacterial sterol transport machinery exists. Until now, the identity of such machinery remained a mystery. In this study, we identified three novel proteins that may be the first examples of transporters for bacterial sterol lipids. The proteins, which all belong to well-studied families of bacterial metabolite transporters, are predicted to reside in the inner membrane, periplasm, and outer membrane of M. capsulatus, and may work as a conduit to move modified sterols to the outer membrane. Quantitative analysis of ligand binding revealed their remarkable specificity for 4-methylsterols, and crystallographic structures coupled with docking and molecular dynamics simulations revealed the structural bases for substrate binding by two of the putative transporters. Their striking structural divergence from eukaryotic sterol transporters signals that they form a distinct sterol transport system within the bacterial domain. Finally, bioinformatics revealed the widespread presence of similar transporters in bacterial genomes, including in some pathogens that use host sterol lipids to construct their cell envelopes. The unique folds of these bacterial sterol binding proteins should now guide the discovery of other proteins that handle this essential metabolite.
引用
收藏
页数:26
相关论文
共 50 条
  • [1] Sterol Synthesis in Diverse Bacteria
    Wei, Jeremy H.
    Yin, Xinchi
    Welander, Paula V.
    FRONTIERS IN MICROBIOLOGY, 2016, 7
  • [2] Intracellular sterol binding proteins: Cholesterol transport and membrane domains
    Schroeder, F
    Frolov, A
    Schoer, JK
    Gallegos, AM
    Atshaves, BP
    Stolowich, NJ
    Scott, AI
    Kier, AB
    INTRACELLULAR CHOLESTEROL TRAFFICKING, 1998, : 213 - 234
  • [3] A novel Tetrahymena thermophila sterol C-22 desaturase belongs to the fatty acid hydroxylase/desaturase superfamily
    Sanchez Granel, Maria L.
    Siburu, Nicolas G.
    Fricska, Annamaria
    Maldonado, Lucas L.
    Gargiulo, Laura B.
    Nudel, Clara B.
    Uttaro, Antonio D.
    Nusblat, Alejandro D.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2022, 298 (10)
  • [4] Bioinformatic analysis of a novel Echinococcus granulosus nuclear receptor with two DNA binding domains
    Alvite, Gabriela
    Riera, Ximena
    Cancela, Saira
    Paulino, Margot
    Esteves, Adriana
    PLOS ONE, 2019, 14 (11):
  • [5] Visualization of Sterol-Rich Membrane Domains with Fluorescently-Labeled Theonellamides
    Nishimura, Shinichi
    Ishii, Kumiko
    Iwamoto, Kunihiko
    Arita, Yuko
    Matsunaga, Shigeki
    Ohno-Iwashita, Yoshiko
    Sato, Satoshi B.
    Kakeya, Hideaki
    Kobayashi, Toshihide
    Yoshida, Minoru
    PLOS ONE, 2013, 8 (12):
  • [6] A novel processing system of sterol regulatory element-binding protein-1c regulated by polyunsaturated fatty acid
    Nakakuki, Masanori
    Kawano, Hiroyuki
    Notsu, Tatsuto
    Imada, Kazunori
    Mizuguchi, Kiyoshi
    Shimano, Hitoshi
    JOURNAL OF BIOCHEMISTRY, 2014, 155 (05) : 301 - 313
  • [7] Sterol methyltransferases in uncultured bacteria complicate eukaryotic biomarker interpretations
    Brown, Malory O.
    Olagunju, Babatunde O.
    Giner, Jose-Luis
    Welander, Paula V.
    NATURE COMMUNICATIONS, 2023, 14 (01)
  • [8] Identification of a novel lipid binding motif in apolipoprotein B by the analysis of hydrophobic cluster domains
    Gordon, Scott M.
    Pourmousa, Mohsen
    Sampson, Maureen
    Sviridov, Denis
    Islam, Rafique
    Perrin, B. Scott, Jr.
    Kemeh, Georgina
    Pastor, Richard W.
    Remaley, Alan T.
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2017, 1859 (02): : 135 - 145
  • [9] Characterization of novel calmodulin binding domains within IQ motifs of IQGAP1
    Jang, Deok-Jin
    Ban, Byungkwan
    Lee, Jin-A
    MOLECULES AND CELLS, 2011, 32 (06) : 511 - 518
  • [10] Identification of Sterol-Containing Domains in Vacuolar Membranes by means of Confocal Microscopy
    Nurminsky, V. N.
    Nesterkina, I. S.
    Spiridonova, E. V.
    Rakevich, A. L.
    Ozolina, N. V.
    BIOLOGICHESKIE MEMBRANY, 2017, 34 (04): : 307 - 312