Raynals, an online tool for the analysis of dynamic light scattering

被引：7

作者：

Burastero, Osvaldo ^{[1
,2
]}

Draper-Barr, George ^{[1
,2
]}

Raynal, Bertrand ^{[3
]}

Chevreuil, Maelenn ^{[3
]}

England, Patrick ^{[3
]}

Alai, Maria Garcia ^{[1
]}

机构：

[1] European Mol Biol Lab, Notkestr 85, Hamburg, Germany

[2] Ctr Struct Syst Biol, Notkestr 85, D-22607 Hamburg, Germany

[3] Univ Paris Cite, CNRS UMR 3528, Inst Pasteur, Plateforme Biophys Mol, Paris, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2023年 / 79卷

关键词：

Raynals; dynamic light scattering; molecular biophysics; online data analysis; protein quality control; hydrodynamic radius; size distribution; software; PROTEIN; OPTIMIZATION; POLYDISPERSITY; DISTRIBUTIONS; NUCLEATION;

D O I：

10.1107/S2059798323004862

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dynamic light scattering (DLS) is routinely employed to assess the homogeneity and size-distribution profile of samples containing microscopic particles in suspension or solubilized polymers. In this work, Raynals, user-friendly software for the analysis of single-angle DLS data that uses the Tikhonov-Phillips regularization, is introduced. Its performance is evaluated on simulated and experimental data generated by different DLS instruments for several proteins and gold nanoparticles. DLS data can easily be misinterpreted and the simulation tools available in Raynals allow the limitations of the measurement and its resolution to be understood. It was designed as a tool to address the quality control of biological samples during sample preparation and optimization and it helps in the detection of aggregates, showing the influence of large particles. Lastly, Raynals provides flexibility in the way that the data are presented, allows the export of publication-quality figures, is free for academic use and can be accessed online on the eSPC data-analysis platform at https://spc.embl-hamburg.de/.

引用

页码：673 / 683

页数：11

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