Anti-inflammatory alkaloids from lotus (Nelumbo nucifera Gaertn.) leaves

被引：2

作者：

Lin, Hong-Yu ^{[1
]}

Chung, Cheng-Pei ^{[2
,3
]}

Hsia, Shih-Min ^{[4
,5
,6
,7
]}

Chang, Yu-Chia ^{[8
]}

Chiang, Wenchang ^{[1
]}

Kuo, Yueh-Hsiung ^{[9
]}

Lin, Yun-Lian ^{[9
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Food Sci & Technol, Taipei 10617, Taiwan

[2] Chang Gung Univ Sci & Technol, Dept Nutr & Hlth Sci, Taoyuan 333324, Taiwan

[3] Chang Gung Univ Sci & Technol, Coll Human Ecol, Res Ctr Food & Cosmet Safety, Taoyuan 333324, Taiwan

[4] Taipei Med Univ, Coll Nutr, Sch Nutr & Hlth Sci, Taipei 110301, Taiwan

[5] Taipei Med Univ Hosp, Nutr Res Ctr, Taipei 110301, Taiwan

[6] Taipei Med Univ, Sch Food & Safety, Taipei 110301, Taiwan

[7] Taipei Med Univ, Grad Inst Metab & Obes Sci, Taipei 110301, Taiwan

[8] Chang Gung Univ Sci & Technol, Coll Human Ecol, Res Ctr Chinese Herbal Med, Taoyuan 333324, Taiwan

[9] China Med Univ, Coll Chinese Med, Dept Chinese Pharmaceut Sci & Chinese Med Resource, Taichung 404327, Taiwan

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2023年 / 249卷 / 03期

关键词：

Nelumbo nucifera Gaertn; leaves; Alkaloids; RAW; 264; 7; macrophages; Anti-inflammation; High-performance liquid chromatography analysis; NITRIC-OXIDE; CONSTITUENTS;

D O I：

10.1007/s00217-022-04169-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Lotus (Nelumbo nucifera Gaertn.) is an aquatic perennial; and lotus leaves were used for food and functional herbal tea in Asia, while alkaloids are regarded as important components. Total alkaloids (TAs), phenolic alkaloids (PAs), and non-PAs from lotus leaves revealed inhibitory effects on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging effects and nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Five alkaloids were isolated and identified as asimilobine (1), roemerine (2), lysicamine (3), nuciferine (4), and N-methylcoclaurine (5). Of them, compounds 1 and 2 exhibited potency as direct NO scavengers while 5 exerted the highest DPPH radical-scavenging effect. Compound 5 also inhibited NO production with an IC50 value of 6 mu M through suppressing inducible NO synthase (iNOS) expression in LPS-induced RAW 264.7 macrophages. Compounds 2 and 3 inhibited NO production (IC50 values of 21 and 25 mu M, respectively) as well as iNOS and cyclooxygenase (COX)-2 expressions. Further, compound 5 occupied 27.5% of TAs by high-performance liquid chromatography (HPLC) analysis, which was mainly responsible for the activities. Besides, no obvious cytotoxicity was observed under treated concentrations. The present study suggests that alkaloid extracts and individual components can be valuable functional ingredients from lotus leaves.

引用

页码：739 / 748

页数：10

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