Oomicidal activity of polypyrrole nanoparticles against Pythium insidiosum

被引：2

作者：

Ianiski, Lara Baccarin ^{[1
]}

Maciel, Aline Fontanella ^{[1
]}

Weiblen, Carla ^{[2
]}

Stibbe, Paula Cristina ^{[1
]}

de Oliveira, Helinando Pequeno ^{[3
]}

da Costa, Mateus Matiuzzi ^{[3
]}

Sangioni, Luis Antonio ^{[1
]}

Pereira, Daniela Isabel Brayer ^{[4
]}

Santurio, Janio Morais ^{[1
]}

Botton, Sonia de Avila ^{[1
,5
]}

机构：

[1] Univ Fed Santa Maria UFSM, BR-97105900 Santa Maria, RS, Brazil

[2] Univ Reg Integrada Alto Uruguai & Missoes URI, BR-97711500 Santiago, RS, Brazil

[3] Univ Vale Sao Francisco UNIVASF, BR-56304205 Petrolina, PE, Brazil

[4] Univ Fed Pelotas UFPel, Inst Biol, BR-96030000 Pelotas, RS, Brazil

[5] Univ Fed Santa Maria, Dept Med Vet Prevent, Ave Roraima n 1000,Predio 44,sala 5132, Santa Maria 97105900RS, CEP, Brazil

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2023年 / 76卷 / 01期

关键词：

zoospores; oomycetes; polypyrrole nanoparticles; pythiosis; Pythium insidiosum; in-vitro susceptibility testing; scanning electron microscopy; IN-VITRO; EQUINE PYTHIOSIS;

D O I：

10.1093/lambio/ovac020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study evaluated in-vitro action of a new molecule, the polypyrrole nanoparticles (Ppy-NP), against Pythium insidiosum isolates using M38-A2/CLSI; the minimal inhibitory (MIC) and minimal oomicidal (MOC) concentrations were also determined. Additionally, changes in the hyphae wall of P. insidiosum CBS 575.85 treated with Ppy-NP were evaluated by scanning electron microscopy (SEM). The MIC100 and MOC for all isolates ranged from 8 to 32 mu g mL(-1), and the MIC90 and MIC50 were 16 mu g mL(-1). The SEM showed structural damage to the hyphae of P. insidisoum treated with Ppy-NP, as hyphae surfaces with less turgidity were found, thereby showing scaling and ruptures compared to the control (untreated hyphae). Our findings highlighted the anti-P. insidiosum properties of Ppy-NP proved to be a promising candidate for research using pythiosis experimental models.

引用

页数：4

共 26 条

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