Interconnection between protein-related chemical functional group spectral features of prairie oat (Avena sativa L.) varieties and ruminant relevant nutrition-Degradation, intestinal digestion and true nutrient supply to dairy cows

被引：1

作者：

Prates, Luciana L. ^{[1
]}

Yu, Peiqiang ^{[1
]}

机构：

[1] Univ Saskatchewan, Coll Agr & Bioresources, Dept Anim & Poultry Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

来源：

JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION | 2023年 / 107卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

chemical functional groups; cool-season oat variety; dairy cows; Prairie; protein structure; rumen degradation and digestion; spectral features; vibrational spectroscopy; DRY-MATTER; BARLEY; STARCH; RUMEN; DEGRADABILITY; CULTIVARS; DVE/OEB; SYSTEM; ENERGY; HULL;

D O I：

10.1111/jpn.13775

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

To our knowledge, the study interconnection between inherent chemical functional group spectral features and nutrient utilisation is still limited. The objective of this study was to test the adequacy of vibrational Fourier transform infrared attenuated total reflectance (ATR-FTIR) spectroscopy as a fast tool to assess the interactive relationship between the nutritive value of the Prairie cool-season oat (Avena sativa L.) varieties in dairy cows and inherent chemical functional group spectral features. The chemical functional group spectral features of the Prairie cool-season oat varieties in western Canada were determined by Fourier transform infrared attenuated total reflectance spectroscopy. The protein-related spectral parameters of chemical functional groups included peak height and peak area intensity of Amide I, Amid II, protein structural alpha-helix and beta-sheet, and their ratios. The rumen degradation kinetics were determined using in situ techniques with four rumen-canulated lactating dairy cows. The intestinal digestion was evaluated using a three-step in vitro technique with 12 h preincubation. The experiment was an randomized complete block design. The data were analysed using the mixed-model procedure of the Statistical Analysis System. The results showed that the interconnection between rumen degradation kinetics, intestinal digestion and true nutrient supply to dairy cows and protein-related chemical functional group spectral features could be revealed by ATR-FTIR with univariate and multi-variate spectral analyses. These findings indicate that ruminant relevant nutritive value of cool-season oats could be rapidly evaluated and predicted using oat-specific functional group spectral characteristics which could be obtained by a non-distractive bioanalytical tool of ATR-FTIR spectroscopy.

引用

页码：783 / 793

页数：11