Confinement effect on the microcapillary flow and shape of red blood cells

被引:1
作者
Nouaman, Mohammed [1 ]
Darras, Alexis [1 ]
Wagner, Christian [1 ,2 ]
Recktenwald, Steffen M. [1 ,3 ]
机构
[1] Saarland Univ, Dept Expt Phys, Dynam Fluids, D-66123 Saarbrucken, Germany
[2] Univ Luxembourg, Phys & Mat Sci Res Unit, L-1511 Luxembourg, Luxembourg
[3] Okinawa Inst Sci & Technol Grad Univ, Micro Bio Nanofluid Unit, 1919-1 Tancha, Onna Son, Okinawa 9040495, Japan
关键词
SHEAR MODULUS; DEFORMATION; DYNAMICS; DEFORMABILITY; TRANSITIONS; MOTION;
D O I
10.1063/5.0197208
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ability to change shape is essential for the proper functioning of red blood cells (RBCs) within the microvasculature. The shape of RBCs significantly influences blood flow and has been employed in microfluidic lab-on-a-chip devices, serving as a diagnostic biomarker for specific pathologies and enabling the assessment of RBC deformability. While external flow conditions, such as the vessel size and the flow velocity, are known to impact microscale RBC flow, our comprehensive understanding of how their shape-adapting ability is influenced by channel confinement in biomedical applications remains incomplete. This study explores the impact of various rectangular and square channels, each with different confinement and aspect ratios, on the in vitro RBC flow behavior and characteristic shapes. We demonstrate that rectangular microchannels, with a height similar to the RBC diameter in combination with a confinement ratio exceeding 0.9, are required to generate distinctive well-defined croissant and slipper-like RBC shapes. These shapes are characterized by their equilibrium positions in the channel cross section, and we observe a strong elongation of both stable shapes in response to the shear rate across the different channels. Less confined channel configurations lead to the emergence of unstable other shape types that display rich shape dynamics. Our work establishes an experimental framework to understand the influence of channel size on the single-cell flow behavior of RBCs, providing valuable insights for the design of biomicrofluidic single-cell analysis applications. (c) 2024 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license(https://creativecommons.org/licenses/by/4.0/).
引用
收藏
页数:10
相关论文
共 50 条
[1]   Start-up shape dynamics of red blood cells in microcapillary flow [J].
Tomaiuolo, Giovanna ;
Guido, Stefano .
MICROVASCULAR RESEARCH, 2011, 82 (01) :35-41
[2]   Numerical simulation of the transient shape of the red blood cell in microcapillary flow [J].
Shi, Xing ;
Wang, Shuanglian ;
Zhang, Shuai .
JOURNAL OF FLUIDS AND STRUCTURES, 2013, 36 :174-183
[3]   Numerical simulation of transient dynamic behavior of healthy and hardened red blood cells in microcapillary flow [J].
Hashemi, Z. ;
Rahnama, M. .
INTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN BIOMEDICAL ENGINEERING, 2016, 32 (11)
[4]   Margination of White Blood Cells in Microcapillary Flow [J].
Fedosov, Dmitry A. ;
Fornleitner, Julia ;
Gompper, Gerhard .
PHYSICAL REVIEW LETTERS, 2012, 108 (02)
[5]   Effect of Cell Age and Membrane Rigidity on Red Blood Cell Shape in Capillary Flow [J].
Nouaman, Mohammed ;
Darras, Alexis ;
John, Thomas ;
Simionato, Greta ;
Rab, Minke A. E. ;
van Wijk, Richard ;
Laschke, Matthias W. ;
Kaestner, Lars ;
Wagner, Christian ;
Recktenwald, Steffen M. .
CELLS, 2023, 12 (11)
[6]   On the shape memory of red blood cells [J].
Cordasco, Daniel ;
Bagchi, Prosenjit .
PHYSICS OF FLUIDS, 2017, 29 (04)
[7]   Shape dynamics of a red blood cell in Poiseuille flow [J].
Agarwal, Dhwanit ;
Biros, George .
PHYSICAL REVIEW FLUIDS, 2022, 7 (09)
[8]   Prediction of noninertial focusing of red blood cells in Poiseuille flow [J].
Hariprasad, Daniel S. ;
Secomb, Timothy W. .
PHYSICAL REVIEW E, 2015, 92 (03)
[9]   Rheology of red blood cells under flow in highly confined microchannels. II. Effect of focusing and confinement [J].
Lazaro, Guillermo R. ;
Hernandez-Machado, Aurora ;
Pagonabarraga, Ignacio .
SOFT MATTER, 2014, 10 (37) :7207-7217
[10]   Microconfined flow behavior of red blood cells [J].
Tomaiuolo, Giovanna ;
Lanotte, Luca ;
D'Apolito, Rosa ;
Cassinese, Antonio ;
Guido, Stefano .
MEDICAL ENGINEERING & PHYSICS, 2016, 38 (01) :11-16