Functional knockout of long non-coding RNAs with genome editing
被引:3
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作者:
Lyu, Qing Rex
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Chongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
Chongqing Acad Sci & Technol, Chongqing, Peoples R ChinaChongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
Lyu, Qing Rex
[1
,2
]
Zhang, Shikuan
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机构:
Tsinghua Shenzhen Int Grad Sch, Key Lab Hlth Sci & Technol Shenzhen, Shenzhen, Peoples R ChinaChongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
Zhang, Shikuan
[3
]
Zhang, Zhe
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机构:
China Japan Friendship Hosp, Dept Chinese Med Gastrointestinal, Beijing 100029, Peoples R ChinaChongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
Zhang, Zhe
[4
]
Tang, Zhiyu
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Chongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R ChinaChongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
Tang, Zhiyu
[1
]
机构:
[1] Chongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China
[2] Chongqing Acad Sci & Technol, Chongqing, Peoples R China
[3] Tsinghua Shenzhen Int Grad Sch, Key Lab Hlth Sci & Technol Shenzhen, Shenzhen, Peoples R China
[4] China Japan Friendship Hosp, Dept Chinese Med Gastrointestinal, Beijing 100029, Peoples R China
An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study.
机构:
Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA
Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA
Liu, S. John
Lim, Daniel A.
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机构:
Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA
Univ Calif San Francisco, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA
San Francisco VA Med Ctr, San Francisco, CA 94121 USAUniv Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA
机构:
Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Lee, Sungwon
Kim, Hyewon
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Kim, Hyewon
Hong, Ari
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Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South Korea
Seoul Natl Univ, Interdisciplinary Program Bioinformat, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Hong, Ari
Song, Jaewon
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Song, Jaewon
Lee, Sungyul
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Lee, Sungyul
Kim, Myeonghwan
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Kim, Myeonghwan
Hwang, Sung-yeon
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Hwang, Sung-yeon
Jeong, Dongjoon
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Jeong, Dongjoon
Kim, Jeesoo
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Kim, Jeesoo
Son, Ahyeon
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Son, Ahyeon
Lee, Young-suk
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Korea Adv Inst Sci & Technol KAIST, Dept Bio & Brain Engn, Daejeon 34141, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Lee, Young-suk
Kim, V. Narry
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Kim, V. Narry
Kim, Jong-seo
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Kim, Jong-seo
Chang, Hyeshik
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机构:
Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South Korea
Seoul Natl Univ, Interdisciplinary Program Bioinformat, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Chang, Hyeshik
Ahn, Kwangseog
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Seoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
Inst for Basic Sci Korea, Ctr RNA Res, Seoul 08826, South KoreaSeoul Natl Univ, Sch Biol Sci, Seoul 08826, South Korea
机构:
Genetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in CairoGenetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in Cairo
Aya El Khodiry
Menna Afify
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Genetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in CairoGenetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in Cairo
Menna Afify
Hend M El Tayebi
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Genetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in CairoGenetic Pharmacology Research Group, Clinical Pharmacy Unit, department of Pharmacology and toxicology, faculty of Pharmacy and biotechnology, German University in Cairo
机构:
Harvard Med Sch, Boston, MA 02115 USAHarvard Med Sch, Boston, MA 02115 USA
Canver, Matthew C.
Bauer, Daniel E.
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机构:
Harvard Med Sch, Boston, MA 02115 USA
Boston Childrens Hosp, Div Hematol Oncol, Boston, MA 02115 USA
Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USAHarvard Med Sch, Boston, MA 02115 USA
Bauer, Daniel E.
Orkin, Stuart H.
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机构:
Harvard Med Sch, Boston, MA 02115 USA
Boston Childrens Hosp, Div Hematol Oncol, Boston, MA 02115 USA
Dana Farber Canc Inst, Dept Pediat Oncol, Boston, MA 02115 USA
Howard Hughes Med Inst, Boston, MA 02115 USAHarvard Med Sch, Boston, MA 02115 USA