Functional knockout of long non-coding RNAs with genome editing

被引：4

作者：

Lyu, Qing Rex ^{[1
,2
]}

Zhang, Shikuan ^{[3
]}

Zhang, Zhe ^{[4
]}

Tang, Zhiyu ^{[1
]}

机构：

[1] Chongqing Gen Hosp, Med Res Ctr, Chongqing, Peoples R China

[2] Chongqing Acad Sci & Technol, Chongqing, Peoples R China

[3] Tsinghua Shenzhen Int Grad Sch, Key Lab Hlth Sci & Technol Shenzhen, Shenzhen, Peoples R China

[4] China Japan Friendship Hosp, Dept Chinese Med Gastrointestinal, Beijing 100029, Peoples R China

来源：

FRONTIERS IN GENETICS | 2023年 / 14卷

关键词：

long non-coding RNA; CRISPR-Cas9; functional knockout; genome editing; methodology; BINDING-PROTEIN; EXPRESSION; TRANSCRIPTION; CRISPR/CAS9; INTERFERENCE;

D O I：

10.3389/fgene.2023.1242129

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An effective loss-of-function study is necessary to investigate the biological function of long non-coding RNA (lncRNA). Various approaches are available, including RNA silencing, antisense oligos, and CRISPR-based genome editing. CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can be achieved by removing the promoter and the first exon (PE1), introducing pre-termination poly(A) signals, or deleting the entire locus, unlike frameshift strategies used for messenger RNA (mRNA). However, the intricate genomic interplay between lncRNA and neighbor genes makes it challenging to interpret lncRNA function accurately. This article discusses the advantages and disadvantages of each lncRNA knockout method and envisions the potential future directions to facilitate lncRNA functional study.

引用

页数：8

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