When Weak Is Strong: A Plea for Low-Affinity Binders for Optical Microscopy

被引：8

作者：

Albertazzi, Lorenzo ^{[1
]}

Heilemann, Mike ^{[2
]}

机构：

[1] Eindhoven Univ Technol, Eindhoven, Netherlands

[2] Goethe Univ Frankfurt, Frankfurt, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2023年 / 62卷 / 35期

关键词：

Exchangeable Fluorophore Labels; Fluorescence Microscopy; Fluorescent Probes; Super-Resolution Microscopy; SUPERRESOLUTION MICROSCOPY; TRACKING; BINDING;

D O I：

10.1002/anie.202303390

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The exploitation of low-affinity molecular interactions in protein labeling is an emerging topic in optical microscopy. Such non-covalent and low-affinity interactions can be realized with various concepts from chemistry and for different molecule classes, and lead to a constant renewal of fluorescence signals at target sites. Further benefits are a versatile use across microscopy methods, in 3D, live and many-target applications. In recent years, several classes of low-affinity labels were developed and a variety of powerful applications demonstrated. Still, this research field is underdeveloped, while the potential is huge.

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页数：6

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共 32 条

[1] Protein labeling for live cell fluorescence microscopy with a highly photostable renewable signal [J].

Bozhanova, Nina G. ;

Baranov, Mikhail S. ;

Klementieva, Natalia V. ;

Sarkisyan, Karen S. ;

Gavrikov, Alexey S. ;

Yampolsky, Ilia V. ;

Zagaynova, Elena V. ;

Lukyanov, Sergey A. ;

Lukyanov, Konstantin A. ;

Mishin, Alexander S. .

CHEMICAL SCIENCE, 2017, 8 (10) :7138-7142

[2] Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes [J].

Carravilla, Pablo ;

Dasgupta, Anindita ;

Zhurgenbayeva, Gaukhar ;

Danylchuk, Dmytro I. ;

Klymchenko, Andrey S. ;

Sezgin, Erdinc ;

Eggeling, Christian .

BIOPHYSICAL REPORTS, 2021, 1 (02)

[3] Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging [J].

Chung, Kenny K. H. ;

Zhang, Zhao ;

Kidd, Phylicia ;

Zhang, Yongdeng ;

Williams, Nathan D. ;

Rollins, Bennett ;

Yang, Yang ;

Lin, Chenxiang ;

Baddeley, David ;

Bewersdorf, Joerg .

NATURE METHODS, 2022, 19 (05) :554-+

[4] Aptamers with Tunable Affinity Enable Single-Molecule Tracking and Localization of Membrane Receptors on Living Cancer Cells [J].

Delcanale, Pietro ;

Porciani, David ;

Pujals, Silvia ;

Jurkevich, Alexander ;

Chetrusca, Andrian ;

Tawiah, Kwaku D. ;

Burke, Donald H. ;

Albertazzi, Lorenzo .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020, 59 (42) :18546-18555

[5] Superresolution Optical Fluctuation Imaging (SOFI) [J].

Dertinger, Thomas ;

Colyer, Ryan ;

Vogel, Robert ;

Heilemann, Mike ;

Sauer, Markus ;

Enderlein, Joerg ;

Weiss, Shimon .

NANO-BIOTECHNOLOGY FOR BIOMEDICAL AND DIAGNOSTIC RESEARCH, 2012, 733 :17-21

[6] Synergizing Exchangeable Fluorophore Labels for Multitarget STED Microscopy [J].

Glogger, Marius ;

Wang, Dongni ;

Kompa, Julian ;

Balakrishnan, Ashwin ;

Hiblot, Julien ;

Barth, Hans-Dieter ;

Johnsson, Kai ;

Heilemann, Mike .

ACS NANO, 2022, 16 (11) :17991-17997

[7] Multi-Color, Bleaching-Resistant Super-Resolution Optical Fluctuation Imaging with Oligonucleotide-Based Exchangeable Fluorophores [J].

Glogger, Marius ;

Spahn, Christoph ;

Enderlein, Joerg ;

Heilemann, Mike .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (12) :6310-6313

[8] Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations [J].

Gustafsson, Nils ;

Culley, Sian ;

Ashdown, George ;

Owen, Dylan M. ;

Pereira, Pedro Matos ;

Henriques, Ricardo .

NATURE COMMUNICATIONS, 2016, 7

[9] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[10] Single-Molecule Kinetics and Super-Resolution Microscopy by Fluorescence Imaging of Transient Binding on DNA Origami [J].

Jungmann, Ralf ;

Steinhauer, Christian ;

Scheible, Max ;

Kuzyk, Anton ;

Tinnefeld, Philip ;

Simmel, Friedrich C. .

NANO LETTERS, 2010, 10 (11) :4756-4761

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