Quantitative Proteomics Reveal the Inherent Antibiotic Resistance Mechanism against Norfloxacin Resistance in Aeromonas hydrophila

被引:7
|
作者
Liu, Ziqiu [1 ,2 ,3 ]
Zhang, Lishan [1 ,2 ,3 ]
Song, Qingli [1 ,2 ,3 ]
Song, Huanhuan [1 ,2 ,3 ]
Xu, Yunqi [1 ,2 ,3 ]
Lu, Jinlian [1 ,2 ,3 ]
Xu, Qiaozhen [1 ,2 ,3 ]
Tang, Yuze [1 ,2 ,3 ]
Liu, Yanling [4 ]
Wang, Guibin [5 ]
Lin, Xiangmin [1 ,2 ,3 ]
机构
[1] Fujian Agr & Forestry Univ, Sch Life Sci, Fujian Prov Key Lab Agroecol Proc & Safety Monitor, Fuzhou 350002, Peoples R China
[2] Fujian Prov Univ, Fujian Agr & Forestry Univ, Key Lab Crop Ecol & Mol Physiol, Fuzhou 350002, Peoples R China
[3] Fujian Agr & Forestry Univ, Inst Oceanol, Key Lab Marine Biotechnol Fujian Prov, Fuzhou 350002, Peoples R China
[4] Fujian Agr & Forestry Univ, Natl Engn Res Ctr JUNCAO Technol, Fuzhou 350002, Peoples R China
[5] Beijing Inst Life, Beijing Proteome Res Ctr, Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing 102206, Peoples R China
关键词
proteomics; Aeromonas hydrophila; norfloxacin; sulfur metabolism; DNA repair; ESCHERICHIA-COLI; PREVALENCE; GENES; FISH; EXPRESSION; PROTEINS; SPP;
D O I
10.1021/acs.jproteome.2c00663
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recently, the prevalence of Aeromonas hydrophila antibiotic-resistant strains has been reported in aquaculture, but its intrinsic antibiotic resistance mechanisms are largely unknown. In the present study, a label-free proteomics technology was used to compare the differential protein abundances in response to norfloxacin (NOR) stress in A. hydrophila. The results showed that there were 186 proteins decreasing and 220 proteins increasing abundances in response to NOR stress. Bioinformatics analysis showed that the differentially expressed proteins were enriched in several biological processes, such as sulfur metabolism and homologous recombination. Furthermore, the antibiotic sensitivity assays showed that the deletion of AHA_0904, cirA, and cysI significantly decreased the resistance against NOR, whereas AAHA_1239, AcysA, AcysD, and AcysN significantly increased the resistance against NOR. Our results provide insights into NOR resistance mechanisms and indicate that AHA_0904, cirA, AHA_1239, and sulfur metabolism may play important roles in NOR resistance in A. hydrophila.
引用
收藏
页码:1193 / 1200
页数:8
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