Physiological response and agronomic performance of drought tolerance mutants of Aus rice cultivar Nagina 22 (Oryza sativa L)

被引:10
作者
Yadav, Neera [1 ]
Sevanthi, Amitha C. M. V. [1 ]
Pandey, Rakesh [2 ]
Chinnusamy, Viswanathan [2 ]
Singh, Ashok K. [2 ]
Singh, Nagendra K. [1 ]
机构
[1] ICAR Natl Inst Plant Biotechnol, New Delhi, India
[2] ICAR Indian Agr Res Inst, New Delhi, India
关键词
Drought tolerance; DRS score; Epicuticular wax; EMS mutants; Rice; CELL-MEMBRANE STABILITY; EPICUTICULAR WAX; CHLOROPHYLL FLUORESCENCE; WATER PERMEABILITY; STRESS; RESISTANCE; BIOSYNTHESIS; SURFACE; GROWTH; ROOTS;
D O I
10.1016/j.fcr.2022.108760
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Rice (Oryza sativa L) is a staple food crop for more than half of the world population, however its yield is adversely affected by drought stress. Traditional rice varieties have significant variability in morphological, physiological and molecular adaptation to drought stress but modern high-yielding cultivars are quite sensitive to drought. Here, we screened a set of 2000 EMS-mutants of a drought tolerant 'Aus' rice cultivar 'Nagina 22 ' (N22) using standard evaluation system of drought sensitivity (DRS) score and identified 73 mutants with either higher or lower DRS score than wild type (WT) N22. The mutants were also screened for epicuticular wax (EW) content to identify 25 mutants with altered levels of EW content, all of which were common to the DRS mutants. The remaining DRS mutants with EW content similar to WT N22 must have other mechanisms of drought tolerance. The identified mutants were evaluated for drought related physiological traits, drought sensitivity index (DSI) and agronomic performance for four consecutive years to understand the role of EW in drought tolerance. All the EW mutants showed altered levels of DSI, biomass and grain yield. The mutants also showed changes in chlorophyll content, relative water content, cell membrane stability and oxidative stress enzyme activities. The high-EW mutants showed lower DRS scores but higher RWC, biomass and grain yield under drought than WT N22, while low-EW mutants showed the opposite trends. The gain and loss of drought tolerance mutants identified here would help gain further insights into the mechanism of drought tolerance and gene discovery for the trait in N22. A high-EW low-DSI mutant viz., 'M59 ' with substantially higher grain yield under drought will serve as useful donor for breeding drought tolerant rice cultivars for rainfed upland agroecological regions.
引用
收藏
页数:14
相关论文
共 50 条
[41]   Biochemical and physiological response of rice (Oryza sativa L.) plants to copper oxide nanoparticle stress [J].
Hassan, Ibrahim A. ;
El Dakak, Rehab ;
Haiha, Nesreen S. ;
Abd Elmegeid, Walaa ;
El-Sheekh, Mostafa ;
Rahman, Salwa Abdul ;
Basahi, Jalal ;
Summan, Ahmad ;
Ismail, Iqbal .
AGROCHIMICA, 2021, 65 (01) :53-67
[42]   Identification of microsatellite markers associated with drought tolerance in rice (Oryza sativa L.) using bulked line analysis [J].
Anitha, R. ;
Saranya, G. ;
Gomez, S. Michael ;
Biji, K. R. ;
Kumar, S. Satheesh ;
Babu, R. Chandra .
PLANT ARCHIVES, 2008, 8 (01) :93-96
[43]   Enhanced Expression of Alternative Oxidase Genes Is Involved in the Tolerance of Rice (Oryza sativa L.) Seedlings to Drought Stress [J].
Feng, Han-Qing ;
Li, Hong-Yu ;
Sun, Kun .
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2009, 64 (9-10) :704-710
[44]   Physiological changes during development of rice (Oryza sativa L.) varieties differing in salt tolerance under saline field condition [J].
Kanawapee, Nantawan ;
Sanitchon, Jirawat ;
Srihaban, Pranee ;
Theerakulpisut, Piyada .
PLANT AND SOIL, 2013, 370 (1-2) :89-101
[45]   Overexpression of Arabidopsis XERICO gene confers enhanced drought and salt stress tolerance in rice (Oryza Sativa L.) [J].
Zeng, De-Er ;
Hou, Pei ;
Xiao, Fangming ;
Liu, Yongsheng .
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 24 (01) :56-64
[46]   Screening of rice landraces (Oryza sativa L.) for seedling stage salinity tolerance using morpho-physiological and molecular markers [J].
Tahjib-Ul-Arif, Md. ;
Abu Sayed, M. ;
Islam, Mirza Mofazzal ;
Siddiqui, M. Nurealam ;
Begum, S. N. ;
Hossain, M. Afzal .
ACTA PHYSIOLOGIAE PLANTARUM, 2018, 40 (04)
[47]   POOLED MAPPING OF QUANTITATIVE TRAIT LOCI ASSOCIATED WITH DROUGHT TOLERANCE IN RICE (ORYZA SATIVA L.) AT SEEDLING STAGE [J].
Wu, Y. B. ;
Zhang, G. Y. ;
Zhang, C. ;
Tang, W. Q. ;
Wang, T. ;
Zhang, H. N. ;
Wu, H. K. .
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS, 2023, 33 (06) :1467-1473
[48]   Effect of fungal biocontrol agents on enhancement of drought stress tolerance in rice (Oryza sativa L.) [J].
Bashyal, Bishnu Maya ;
Zaidi, Najam Waris ;
Singh, Uma Shankar ;
Aggarwal, Rashmi .
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 2020, 57 (01) :101-108
[49]   Identification of quantitative trait loci associated with drought tolerance traits in rice (Oryza sativa L.) under PEG and field drought stress [J].
Han, Bing ;
Wang, Jiao ;
Li, Yafei ;
Ma, Xiaoding ;
Jo, Sumin ;
Cui, Di ;
Wang, Yanjie ;
Park, Dongsoo ;
Song, Youchun ;
Cao, Guilan ;
Wang, Xingsheng ;
Sun, Jianchang ;
Shin, Dongjin ;
Han, Longzhi .
EUPHYTICA, 2018, 214 (04)
[50]   Comparative Metabolomics Combined with Physiological Analysis Revealed Cadmium Tolerance Mechanism in Indica Rice (Oryza sativa L.) [J].
Chang, Weixia ;
Wang, Wei ;
Shi, Zhangsheng ;
Cao, Guodong ;
Zhao, Xingchen ;
Su, Xiuli ;
Chen, Yi ;
Wu, Jiabin ;
Yang, Zhu ;
Liu, Chaolei ;
Shang, Lianguang ;
Cai, Zongwei .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 71 (20) :7669-7678