Establishment of an efficient early flowering-assisted CRISPR/Cas9 gene-editing system in Arabidopsis

被引：3

作者：

Lao, Kangwen ^{[1
]}

Xiao, Yinlin ^{[2
]}

Huang, Qing ^{[1
]}

Mo, Beixin ^{[2
]}

Dong, Xianxin ^{[3
]}

Wang, Xiaoyan ^{[1
]}

机构：

[1] Beijing Normal Univ, Ctr Biol Sci & Technol, Adv Inst Nat Sci, Zhuhai 519087, Peoples R China

[2] Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China

[3] Beijing Normal Univ, Instrumentat & Serv Ctr Sci & Technol, Zhuhai 519087, Peoples R China

来源：

PLANT CELL REPORTS | 2023年 / 42卷 / 01期

关键词：

CRISPR; Cas9; FLOWERING LOCUS T; Early-flowering; DsRed; Gene editing;

D O I：

10.1007/s00299-022-02944-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Key message Ectopic expression of the florigen FT gene along with the Cas9 cassette promotes fast reproduction of the T1 transgenic plants, and the late-flowering phenotype serves as a convenient marker for transgene-free plants screening in T2 segregation population.

引用

页码：211 / 214

页数：4

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