Exposure to dietary fatty acids oleic and palmitic acid alters structure and mechanotransduction of intestinal cells in vitro

被引:6
|
作者
Bergen, Janice [1 ,2 ]
Karasova, Martina [1 ,2 ]
Bileck, Andrea [3 ,4 ]
Pignitter, Marc [5 ]
Marko, Doris [1 ]
Gerner, Christopher [3 ,4 ]
Del Favero, Giorgia [1 ,2 ]
机构
[1] Univ Vienna, Fac Chem, Dept Food Chem & Toxicol, Wahringerstr 38-42, A-1090 Vienna, Austria
[2] Univ Vienna, Fac Chem, Core Facil Multimodal Imaging, Wahringerstr 38-42, A-1090 Vienna, Austria
[3] Univ Vienna, Fac Chem, Dept Analyt Chem, Wahringerstr 38-42, A-1090 Vienna, Austria
[4] Univ Vienna, Med Univ Vienna, Joint Metabolome Facil, Vienna, Austria
[5] Univ Vienna, Fac Chem, Dept Physiol Chem, Josef Holaubek Pl 2, A-1090 Vienna, Austria
关键词
Intestinal toxicity; HCT116; HCEC-1CT; Palmitic acid; Oleic acid; Mechanosensory apparatus; TETRAZOLIUM SALT; TUMOR-SUPPRESSOR; PLATE ASSAY; ACTIN; YAP/TAZ; TRANSDUCTION; CYTOSKELETON; ASSOCIATION; DOWNSTREAM; PATHWAYS;
D O I
10.1007/s00204-023-03495-3
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Intestinal cells are continuously exposed to food constituents while adapting to peristaltic movement and fluid shear stress. Oleic acid (OA) and palmitic acid (PA) are among the most prevalent fatty acids with respect to dietary lipids. Despite the central importance of dietary lipids for a balanced diet, awareness about potential detrimental effects related to excessive consumption is increasing; this includes toxicity, metabolic deregulation, and, particularly for cancer cells, a benefit from the uptake of fatty acids related to promotion of metastasis. Expanding on this, we started elucidating the effects of OA and PA (25-500 mu M) on non-transformed human intestinal epithelial cells (HCEC-1CT) in comparison to colon carcinoma cells (HCT116), with regard to the mechanosensory apparatus. Hence, intestinal cells' motility is on the one side essential to ensure adaption to peristaltic movement and barrier function, but also to enable metastatic progression. Incubation with both OA and PA (>= 25 mu M) significantly decreased membrane fluidity of HCT116 cells, whereas the effect on HCEC-1CT was more limited. Application of rhodamine-labelled PA demonstrated that the fatty acid is incorporated into the plasma membrane of HCT116, which could not be observed in the non-tumorigenic cell line. Down-streaming into the intracellular compartment, a pronounced rearrangement of actin cytoskeleton was evident in both cell lines (OA and PA; 25 and 100 mu M). This was accompanied by a variation of translocation efficiency of the mechanosensitive co-transcription factor YAP1, albeit with a stronger effect seen for PA and the cancer cells. Untargeted proteomic analysis confirmed that exposure to OA and PA could alter the response capacity of HCT116 cells to fluid shear stress. Taken together, OA and PA were able to functionally modulate the mechanosensory apparatus of intestinal cells, implying a novel role for dietary fatty acids in the regulation of intestinal pathophysiology.
引用
收藏
页码:1659 / 1675
页数:17
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