Identification and analysis of long non-coding RNAs that are involved in response to GCRV infection in grass carp (Ctenopharyngodon idella)

被引:2
|
作者
Zhang, Yexuan [1 ,2 ]
Hu, Xudong [1 ,2 ]
Liu, Shuai [3 ]
Zhou, Man [1 ,2 ]
Wang, Chunling [1 ,2 ]
Cao, Hong [1 ,2 ]
机构
[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Dalian Ocean Univ, Coll Fishery & Life Sci, Dalian 116023, Peoples R China
基金
中国国家自然科学基金;
关键词
Grass carp; Grass carp reovirus (GCRV); C; idella kidney (CIK) cells; lncRNA; INNATE; TRANSCRIPTS; EVOLUTION; LINCRNAS;
D O I
10.1016/j.fsi.2023.108623
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Long noncoding RNAs (lncRNAs) play important roles in many biological processes including the immune response against virus infection. However, their roles in grass carp reovirus (GCRV) pathogenicity are largely unknown. In this study, the next-generation sequencing (NGS) technology was used to analyze the profiles of lncRNAs in GCRV-infected and mock-infected grass carp kidney (CIK) cells. Our results showed that 37 lncRNAs and 1039 mRNA transcripts exhibited differential expression in CIK cells after GCRV infection compared with the mock infection. Functional analysis through the gene ontology and Kyoto Encyclopedia of Genes and Genomes databases (KEGG) indicated that target genes of the differentially expressed lncRNAs were mainly enriched in the biological processes -biological regulation, cellular process, metabolic process and regulation of the biological process, such as MAPK signaling pathway and Notch signaling. Furthermore, we observed that the lncRNA3076 (ON693852) was markedly upregulated after the GCRV infection. In addition, silencing lncRNA3076 decreased the GCRV replication, which indicates that it might play an important role in the replication of GCRV.
引用
收藏
页数:8
相关论文
共 50 条
  • [21] Genetic structure, polymorphism identification of LGP2 gene and their relationship with the resistance/susceptibility to GCRV in grass carp, Ctenopharyngodon idella
    Wan, Quanyuan
    Wang, Lan
    Su, Jianguo
    Yang, Chunrong
    Peng, Limin
    Chen, Lijun
    GENE, 2013, 521 (01) : 166 - 175
  • [22] Long non-coding RNAs involved in retinoblastoma
    Nasrolahi, Ava
    Azizidoost, Shirin
    Radoszkiewicz, Klaudia
    Najafi, Sajad
    Ghaedrahmati, Farhoodeh
    Sheykhi-Sabzehpoush, Mohadeseh
    Poodineh, Jafar
    Hajizadeh, Maryam
    Anbiyaiee, Amir
    Farzaneh, Maryam
    Feghhi, Mostafa
    JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2023, 149 (01) : 401 - 421
  • [23] Long non-coding RNAs involved in retinoblastoma
    Ava Nasrolahi
    Shirin Azizidoost
    Klaudia Radoszkiewicz
    Sajad Najafi
    Farhoodeh Ghaedrahmati
    Mohadeseh Sheykhi-Sabzehpoush
    Jafar Poodineh
    Maryam Hajizadeh
    Amir Anbiyaiee
    Maryam Farzaneh
    Mostafa Feghhi
    Journal of Cancer Research and Clinical Oncology, 2023, 149 : 401 - 421
  • [24] Identification of long non-coding RNAs in response to microsporidia infection in Silkworm, Bombyx mori
    Pu, Shiqi
    Fang, Yujia
    Yang, Yuhang
    Qu, Qingsheng
    Liu, Mengjin
    Lian, Jialin
    Tang, Xudong
    Shen, Zhongyuan
    Qian, Ping
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2024, 117 (03) : 772 - 781
  • [25] Autophagy Inhibits Grass Carp Reovirus (GCRV) Replication and Protects Ctenopharyngodon idella Kidney (CIK) Cells from Excessive Inflammatory Responses after GCRV Infection
    Chu, Pengfei
    He, Libo
    Huang, Rong
    Liao, Lanjie
    Li, Yongming
    Zhu, Zuoyan
    Hu, Wei
    Wang, Yaping
    BIOMOLECULES, 2020, 10 (09) : 1 - 23
  • [26] Identification of multifunctionality of grass carp (Ctenopharyngodon idella) TBK1 during bacterial infection
    Yu, Hongyan
    Jia, Xuewen
    Pang, Yifan
    Niu, Huiqin
    Du, Biao
    Xu, Xiaoyan
    Li, Jiale
    FISH & SHELLFISH IMMUNOLOGY, 2023, 136
  • [27] Molecular cloning and expression analysis of coagulation factor VIII and plasminogen involved in immune response to GCRV, and immunity activity comparison of grass carp Ctenopharyngodon idella with different viral resistance
    Wang, Hongquan
    Ding, Chunhua
    Wang, Jing'an
    Zhao, Xin
    Jin, Shengzhen
    Liang, Jian
    Luo, Hong
    Li, Dongfang
    Li, Rui
    Li, Yaoguo
    Xiao, Tiaoyi
    FISH & SHELLFISH IMMUNOLOGY, 2019, 86 : 794 - 804
  • [28] Identification, characterization and immunological response analysis of stimulator of interferon gene (STING) from grass carp Ctenopharyngodon idella
    Feng, Xiaoli
    Yang, Chunrong
    Zhang, Yixuan
    Peng, Limin
    Chen, Xiaohui
    Rao, Youliang
    Gu, Tianle
    Su, Jianguo
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2014, 45 (01): : 163 - 176
  • [29] Matrix metalloproteinase 2 of grass carp Ctenopharyngodon idella (CiMMP2) is involved in the immune response against bacterial infection
    Xu, Xiao-Yan
    Shen, Yu-Bang
    Fu, Jian-Jun
    Liu, Feng
    Guo, Shi-Zhao
    Yang, Xiao-Meng
    Li, Jia-Le
    FISH & SHELLFISH IMMUNOLOGY, 2012, 33 (02) : 251 - 257
  • [30] The effects of GCRV on various tissues of grass carp ( Ctenopharyngodon idella) ) and identification of differential interferon-stimulating genes (ISGs) through muscle transcriptome analysis
    Yang, Ziquan
    Zhang, Huimei
    Yuan, Ziming
    Chen, Jie
    Zheng, Guodong
    Zou, Shuming
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2024, 284