Phytofabrication of silver and zinc oxide nanoparticles using the fruit extract of Phyllanthus emblica and its potential anti-diabetic and anti-cancer activity

Phytochemicals capacity to reduce metal into nanoparticles has sparked a new eco-friendly method to build green nanotechnology. Synthesis of nanoparticles using a biological approach with valuable medicinal applications is an essential feature of modern nanotechnology. The present study attempts to synthesize silver (PE-AgNPs) and zinc oxide (PE-ZnONPs) nanoparticles using Phyllanthus emblica fruit ethanolic extract and evaluates their therapeutic potential. In our present study, the secondary metabolites of the extract were determined by Gas Chromatography-Mass Spectra (GC-MS.) Nanoparticles were characterized using analytical tools. The PE-AgNPs and PE-ZnONPs nanoparticles have shown potential inhibition activity against alpha-amylase (IC50 170.60 +/- 0.94 and 160.82 +/- 0.88 mu g/mL), alpha-glucosidase (IC50 80.24 +/- 1.38 and 70.02 +/- 0.59 mu g/mL) and increased glucose uptake (180.42 +/- 0.78 and 110.52 +/- 0.84 mu g/mL). In-vitro MTT assay of PE-AgNPs and PE-ZnONPs nanoparticles revealed the effective anticancer potential against the A549 cancer cell line with an IC50 value of 27.52 +/- 1.12 and 70.64 +/- 1.4 mu g/mL. The synthesized bioactive compounds can be the alternative eco-friendly potential for an antidiabetic and anticancer substitute which can be further recommended for pharmacological applications.