Influence of microplastic-associated biofilms on the bioavailability of a mixture of cadmium and benzo[a]pyrene by the analysis of biomarker gene expression in larval zebrafish

被引：4

作者：

Di Natale, Marilena ^{[1
,2
]}

Catarino, Ana Isabel ^{[3
,4
]}

Summers, Stephen ^{[5
]}

Boyle, David ^{[3
]}

Torri, Marco ^{[1
]}

Nicosia, Aldo ^{[6
]}

Musco, Marianna ^{[1
,7
]}

Masullo, Tiziana ^{[1
]}

Russo, Stefania ^{[1
]}

Bennici, Carmelo Daniele ^{[1
]}

Mazzola, Antonio ^{[2
]}

Cuttitta, Angela ^{[1
,7
]}

Henry, Theodore B. ^{[3
]}

机构：

[1] CNR, Natl Res Council Italy, Detached Unit Palermo, Inst Studies Mediterranean ISMed, Via Filippo Parlatore 65, I-90145 Palermo, Italy

[2] Univ Palermo, Dept Earth & Marine Sci, Via Archirafi 22, I-90123 Palermo, PA, Italy

[3] Heriot Watt Univ, Inst Life & Earth Sci, Sch Energy Geosci Infrastruct & Soc, Edinburgh EH144AS, Scotland

[4] Flanders Marine Inst VLIZ, InnovOcean Campus,Jacobsenstr 1, B-8400 Oostende, Belgium

[5] Natl Univ Singapore, Singapore Ctr Environm Life Sci Engn, Singapore 119077, Singapore

[6] CNR, Natl Res Council Italy, Inst Biomed Res & Innovat IRIB, Via Ugo Malfa, 153, I-90146 Palermo, Italy

[7] LUMSA Univ, Via Filippo Parlatore 65, Palermo, Italy

来源：

ECOLOGICAL INDICATORS | 2023年 / 152卷

基金：

英国自然环境研究理事会;

关键词：

Microplastics; Biofilm; Contaminants; Mixture; Zebrafish; Sorption; Bioavailability; COMPETITIVE SORPTION; CHEMICALS; INDUCTION; SULFAMETHOXAZOLE; ENVIRONMENT; COEXPOSURE; TRANSPORT; KINETICS; PLASTICS;

D O I：

10.1016/j.ecolind.2023.110369

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Microplastics (MPs, 1 mu m - 5 mm) in aquatic environments undergo complex weathering transformations such as those induced by microbial colonization and biofilm formation, that affect their ability to interact with envi-ronmental contaminants (co-contaminants). In this study, the microbial composition of MP biofilms and its in-fluence on the sorption and bioavailability of two co-contaminants with different physicochemistry, benzo[a] pyrene (B[a]P) and cadmium (Cd) in a mixture, were assessed. Aqueous-phase bioavailability was measured by assessment of biomarker gene expression for these toxicants (cytochrome P450 1A, cyp1a, and metallothionein 2, mt2, for B[a]P and Cd respectively) in larval zebrafish, Danio rerio. Significant induction of cyp1a and mt2 gene expression (p < 0.05) was observed after exposure to the mixture of Cd, B[a]P and MPs compared to joint exposure with individual contaminants and MPs. Significant changes in bioavailability for mt2 biomarker (p < 0.001) resulted after exposure to a Cd and B[a]P mixture with MPs compared to the same exposure without MPs. Biofilms significantly reduced bioavailability of B[a]P (cyp1a gene expression (p < 0.01)) but not Cd (mt2 gene expression) in the mixture with Cd and B[a]P (HDPE + BF + B[a]P + Cd) compared to the same treatment without biofilm (HDPE + B[a]P + Cd). Thus, compared to Cd, the biofilm could provide additional interactions with B[a]P, and new specific active sites on the MPs surface, that reduced B[a]P bioavailability. Additionally, the biofilm microbial community included hydrocarbon-degrading bacteria able to metabolize hydrophobic chem-icals. These data indicated that in a mixture of co-contaminants, the biofilm selectively influenced their bioavailability and that the microbial composition of MPs biofilm may have played a key role in reducing B[a]P bioavailability. The results of this study highlight how in a complex exposure scenario characterized by a mixture of different co-contaminants, the polymer and chemical properties and micro-surroundings of the organisms may affect contaminants' bioavailability and/or exposure.

引用

页数：12

共 78 条

[31]

Koelmans A., 2021, Weight of evidence for the microplastic vector effect in the context of chemical risk assessment, P155, DOI [DOI 10.1007/978-3-030-78627-4_6, 10.1007/978-3-030-78627-4_6]

[32] Sorption capacity of plastic debris for hydrophobic organic chemicals [J].