In situ visualization of Braun?s lipoprotein on E. coli sacculi

被引：3

作者：

Sheng, Qi ^{[1
,2
,3
]}

Zhang, Meng-Yao ^{[1
]}

Liu, Si -Min ^{[1
]}

Chen, Zhuo-Wei ^{[1
]}

Yang, Pei-Ling ^{[1
]}

Zhang, Hong-Su ^{[1
]}

Liu, Meng-Yun ^{[1
]}

Li, Kang ^{[1
,4
]}

Zhao, Long-Sheng ^{[1
]}

Liu, Ning-Hua ^{[1
]}

Liu, Lu-Ning ^{[2
,3
,5
]}

Chen, Xiu-Lan ^{[1
,4
]}

Hobbs, Jamie K. ^{[4
,6
,7
]}

Foster, Simon J. ^{[4
,6
,8
]}

Zhang, Yu-Zhong ^{[2
,3
,4
,9
]}

Su, Hai-Nan ^{[1
,4
]}

机构：

[1] Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China

[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[3] Ocean Univ China, Frontiers Sci Ctr Deep Ocean Multispheres & Earth, Qingdao 266003, Peoples R China

[4] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China

[5] Univ Liverpool, Inst Syst Mol & Integrat Biol, Liverpool, England

[6] Univ Sheffield, Florey Inst Host Pathogen Interact, Sheffield, England

[7] Univ Sheffield, Dept Phys & Astron, Sheffield, England

[8] Univ Sheffield, Sch Biosci, Sheffield, England

[9] Shandong Univ, Marine Biotechnol Res Ctr, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China

来源：

SCIENCE ADVANCES | 2023年 / 9卷 / 03期

基金：

中国国家自然科学基金; 国家重点研发计划; 英国惠康基金;

关键词：

OUTER-MEMBRANE LIPOPROTEIN; ATOMIC-FORCE MICROSCOPY; ESCHERICHIA-COLI; MUREIN-LIPOPROTEIN; CELL-WALL; PROTEIN; PEPTIDOGLYCAN; PAL; LOCALIZATION; ATTACHMENT;

D O I：

10.1126/sciadv.add8659

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Braun's lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in Escherichia coli, as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important chal-lenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope-associated protein. Collectively, we have established a framework to elucidate the distri-bution of Lpp and other peptidoglycan-bound proteins via a direct imaging modality.

引用

页数：9

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