Amorphous Fe-Containing Phosphotungstates Featuring Efficient Peroxidase-like Activity at Neutral pH: Toward Portable Swabs for Pesticide Detection with Tandem Catalytic Amplification

被引:51
|
作者
Zhu, Hengjia [1 ,2 ]
Liu, Bangxiang [2 ]
Wang, Mengzhu [2 ]
Pan, Jianming [2 ]
Xu, Lizhang [1 ]
Hu, Panwang [2 ]
Niu, Xiangheng [1 ,2 ,3 ]
机构
[1] Jiangsu Univ, Sch Agr Engn, Zhenjiang 212013, Peoples R China
[2] Jiangsu Univ, Inst Green Chem & Chem Technol, Sch Chem & Chem Engn, Zhenjiang 212013, Peoples R China
[3] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hengyang 421001, Peoples R China
基金
中国国家自然科学基金;
关键词
ENZYMATIC CASCADE REACTIONS; METAL-ORGANIC FRAMEWORKS; GRAPHENE OXIDE; NANOZYME; GLUCOSE; ACID; NANOPARTICLES; ATP;
D O I
10.1021/acs.analchem.3c00008
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Peroxidase-mimetic materials are intensively applied to establish multienzyme systems because of their attractive merits. However, almost all of the nanozymes explored exhibit catalytic capacity only under acidic conditions. The pH mismatch between peroxidase mimics in acidic environments and bioenzymes under neutral conditions significantly restricts the development of enzyme-nanozyme catalytic systems especially for biochemical sensing. To solve this problem, here amorphous Fe-containing phosphotungstates (Fe-PTs) featuring high peroxidase activity at neutral pH were explored to fabricate portable multienzyme biosensors for pesticide detection. The strong attraction of negatively charged Fe-PTs to positively charged substrates as well as the accelerated regeneration of Fe2+ by the Fe/W bimetallic redox couples was demonstrated to play important roles in endowing the material with peroxidase-like activity in physiological environments. Consequently, integrating the developed Fe-PTs with acetylcholinesterase and choline oxidase led to an enzyme- nanozyme tandem platform with good catalytic efficiency at neutral pH for organophosphorus pesticide response. Furthermore, they were immobilized onto common medical swabs to fabricate portable sensors for paraoxon detection conveniently based on smartphone sensing, showing excellent sensitivity, good anti-interference capacity, and low detection limit (0.28 ng/mL). Our contribution expands the horizon of acquiring peroxidase activity at neutral pH, and it will also open avenues to construct portable and effective biosensors for pesticides and other analytes.
引用
收藏
页码:4776 / 4785
页数:10
相关论文
共 4 条
  • [1] Amorphous iron-catecholates featuring efficient peroxidase-like activity for quick colorimetric detection of tannic acid
    Wu, Cunhui
    Qin, Zhiyang
    Liu, Yixin
    Qin, Xinguang
    Liu, Gang
    Wei, Xinlin
    Zhang, Haizhi
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2024, 197
  • [2] Coordinated carbon dots-Fe featuring synergistically enhanced peroxidase-like activity at neutral pH for specific and background-free detection of dichlorvos
    Yang, Yangyu
    Xu, Jinju
    Zhou, Rongrong
    Qin, Ziyi
    Liao, Chunhui
    Shi, Shuyun
    Chen, Yuxia
    Guo, Ying
    Zhang, Shuihan
    CARBON, 2024, 219
  • [3] Highly efficient colorimetric detection of cancer cells utilizing Fe-MIL-101 with intrinsic peroxidase-like catalytic activity over a broad pH range
    Chen, Daomei
    Li, Bin
    Jiang, Liang
    Duan, Deliang
    Li, Yizhou
    Wang, Jiaqiang
    He, Jiao
    Zeng, Yanbo
    RSC ADVANCES, 2015, 5 (119): : 97910 - 97917
  • [4] Co-catalytic enhancement of peroxidase-like activity of Fe-MOFs nanozymes by AuNPs for efficient specific detection of Hg2+
    Ren, Yue
    Liao, Zixiang
    He, Yi
    Li, Libo
    You, Tianyan
    MICROCHEMICAL JOURNAL, 2025, 208