Characterization of a Group of 2,3-Oxidosqualene Cyclase Genes Involved in the Biosynthesis of Diverse Triterpenoids of Perilla frutescens

被引：7

作者：

Huang, Ruoshi ^{[1
]}

Wu, Duan ^{[2
]}

Ji, Zhongju ^{[1
]}

Fan, Baolian ^{[1
]}

She, Yaru ^{[1
]}

Zhang, Xiande ^{[1
]}

Duan, Lixin ^{[1
]}

Shen, Qi ^{[2
]}

机构：

[1] Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Sch Pharmaceut Sci, Joint Int Res Lab Translat Canc Res Chinese Med,Gu, Guangzhou 510006, Peoples R China

[2] Guangzhou Univ Chinese Med, Inst Med Plant Physiol & Ecol, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 05期

基金：

中国国家自然科学基金;

关键词：

2; 3-oxidosqualene cyclases (OSCs); Perilla frutescens; triterpene biosynthesis; camelliol C; CYCLOARTENOL SYNTHASE; STERIC BULK; EXPRESSION; ALCOHOLS; CLONING; LEAVES;

D O I：

10.1021/acs.jafc.2c07716

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Perilla frutescens (L.), a traditional edible and medicinal crop, contains diverse triterpenes with multiple pharmacological properties. However, the biosynthesis of triterpenes in perilla remains rarely revelation. In this study, nine putative 2,3-oxidosqualene cyclase (OSC) genes (PfOSC1-9) were screened from the P. frutescens genome and functionally characterized by heterologous expression. Camelliol C, a triterpenol with pharmacological effect, was first identified as abundant in perilla seeds, and the camelliol C synthase (PfOSC7) was first identified in P. frutescens utilizing a yeast system. In addition, PfOSC2, PfOSC4, and PfOSC9 were identified as cycloartenol, lupeol, and beta-amyrin synthase, respectively. Molecular docking and site directed mutagenesis revealed that changes in Leu253 of PfOSC4, Ala480 of PfOSC7, and Trp257 of PfOSC9 might lead to variations of catalytic specificity or efficiency. These results will provide key insights into the biosynthetic pathways of triterpenoids and have great significance for germplasm breeding in P. frutescens.

引用

页码：2523 / 2531

页数：9

共 39 条

[1] MOLECULAR-CLONING, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF RAT OXIDOSQUALENE CYCLASE CDNA [J].