Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

被引：29

作者：

Gu, Binbin ^{[1
]}

Goldfuss, Bernd ^{[2
]}

Dickschat, Jeroen S. S. ^{[1
]}

机构：

[1] Univ Bonn, Kekule Inst Organ Chem & Biochem, Gerhard Domagk Str 1, D-53121 Bonn, Germany

[2] Univ Cologne, Dept Chem, Greinstr 4, D-50939 Cologne, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2023年 / 62卷 / 01期

关键词：

Biosynthesis; Enzyme Engineering; Enzyme Mechanisms; Isotopes; Terpenoids; GERANYL PYROPHOSPHATE; BIOSYNTHESIS; CYCLIZATION; PRENYLTRANSFERASE; DIPHOSPHATE; ENZYMES;

D O I：

10.1002/anie.202215688

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme engineering through site-directed mutagenesis gave access to a high-yielding enzyme variant that provided six additional minor products and the main product in sufficient quantities to study its chemistry.

引用

页数：6

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