Physical and antibacterial properties of Chitosan-guar-peppermint gel for improving wound healing

Burn wound healing can result in complex problems; therefore, the discovery of an effective method to improve the healing process is vital. Chitosan is applied as a natural biopolymer for wound healing because of its non-toxicity activity, biodegradability, and biocompatibility. Natural metabolites would possibly function like anti-inflammatory and antimicrobial agents and speed up the curing procedure. Using antibacterial wound dressings can efficiently prevent the leftover antibacterial nanomaterials for injection in vivo, thus their biological toxicity to common tissues and cells decreases, causing them to be greatly administered in the biomedical field. The present research intends to evaluate the healing potential of antibacterial guar gum/chitosan/peppermint essential oil hydrogel in the healing of full-thickness burns in a rat model. After creating a complete thickness burn Staphylococcus sp, infected wound on each rat dorsum under general anesthesia, the wound burns were healed with chitosan/guar gum hydrogel; chitosan gel; chitosan/Peppermint hydrogel; chitosan/guar gum/eppermint hydrogel, respectively. Also, the results of the minimum inhibitory concentration of gel for Candida glabrata, Candida Albicans, Escherichia coli and Staphylococcus sp obtained 9.2, 8.3, 7.6, and 5.7 mg/ml respectively. The histopathological and histomorphological observations, including wound contraction, epithelial gap, angiogenesis, the thickness of collagen fibers, fibroblast cells agglomeration, hyperemia, and inflammatory cellular reaction revealed that chitosan/guar gum/peppermint had the best function in the wound healing process (On the 22nd day, the rate of wound contraction was about 90%.) Excellent performance of chitosan/guar gum/Peppermint hydrogel in the healing process provides state-of-the-art knowledge about manufacturing clinical antibacterial wound curing goods.