A Proximity-Induced Fluorogenic Reaction Triggered by Antibody-Antigen Interactions with Adjacent Epitopes

被引：1

作者：

Nishiyama, Kentaro ^{[1
]}

Akiba, Hiroki ^{[1
,2
]}

Nagata, Satoshi ^{[2
]}

Tsumoto, Kouhei ^{[2
,3
]}

Kamada, Haruhiko ^{[1
,2
]}

Ohno, Hiroaki ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan

[2] Natl Inst Biomed Innovat Hlth & Nutr, Ibaraki, Osaka 5670085, Japan

[3] Univ Tokyo, Sch Engn, Tokyo 1138656, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2023年 / 62卷 / 30期

关键词：

Antibodies; Bioconjugation; Click Chemistry; Epitope; Template Synthesis; CYCLOADDITION; MIRNA;

D O I：

10.1002/anie.202306431

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Proximity-induced chemical reactions are site-specific and rapid by taking advantage of their high affinity and highly selective interactions with the template. However, reactions induced solely by antibody-antigen interactions have not been developed. Herein, we propose a biepitopic antigen-templated chemical reaction (BATER) as a novel template reaction. In BATER, reactive functional groups are conjugated to two antibodies that interact with two epitopes of the same antigen to accelerate the reaction. We developed a method for visualizing the progress of BATER using fluorogenic click chemistry for optimal antibody selection and linker design. The reaction is accelerated in the presence of a specific antigen in a linker length-dependent manner. The choice of the antibody epitope is important for a rapid reaction. This design will lead to various applications of BATER in living systems.

引用

页数：5