Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

被引：1
|
作者：
Adams, Alexandra C. [1 ]
Kufcsak, Andras [1 ]
Ehrlich, Katjana [3 ]
Dhaliwal, Kevin [1 ]
Seth, Sohan [1 ,2 ,3 ]
机构：
[1] Univ Edinburgh, Queens Med Res Inst, Ctr Inflammat Res, Translat Healthcare Technol Grp, Edinburgh EH16 4TJ, Scotland
[2] Univ Edinburgh, Sch Informat, Edinburgh EH16 4TJ, Scotland
[3] Heriot Watt Univ, Scottish Univ Phys Alliance SUPA, Edinburgh EH16 4TJ, Scotland
来源：
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING | 2023年 / 70卷 / 08期
基金：
英国医学研究理事会;
关键词：
Fluorescence lifetime; time-resolved fluor-escence spectroscopy; B-splines; non-linear least squares; OF-MASS METHOD; LIFETIME STANDARDS; SPECTROSCOPY; YIELDS; DECAY;
D O I：
10.1109/TBME.2023.3244664
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.
引用
收藏
页码：2395 / 2403
页数：9
相关论文
共 50 条
  • [11] Multispectral excitation of time-resolved fluorescence of biological compounds: Variation of fluorescence lifetime with excitation and emission wavelengths
    Maarek, JMI
    Kim, S
    ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY V, 2001, 4252 : 124 - 131
  • [12] Spectral and time-resolved analysis of light-induced changes of NADPH fluorescence in chloroplasts
    Latouche, G
    Montagnini, F
    Cerovic, ZG
    Moya, I
    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, 1998, : 4349 - 4352
  • [13] Sensitivity of Time-Resolved Fluorescence Analysis Methods for Disease Detection
    Agrawal, Anant
    Gallas, Brandon D.
    Parker, Camisha
    Agrawal, Krishan M.
    Pfefer, T. Joshua
    IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, 2010, 16 (04) : 877 - 885
  • [14] A novel method for detection of camellia oil adulteration based on time-resolved emission fluorescence
    Chen, Hui
    Chen, Bin
    Lu, Daoli
    SCIENTIFIC REPORTS, 2018, 8
  • [15] Novel setup for time-resolved fluorescence microscopy
    Gundlach, Lars
    Piotrowiak, Piotr
    PHYSICAL CHEMISTRY OF INTERFACES AND NANOMATERIALS VI, 2007, 6643
  • [16] Ultrafast time-resolved fluorescence at cryogenic temperature
    Son, Jiwon
    Joo, Taiha
    REVIEW OF SCIENTIFIC INSTRUMENTS, 2018, 89 (08)
  • [17] Time-resolved fluorescence spectroscopy for chemical sensors
    Draxler, S
    Lippitsch, ME
    APPLIED OPTICS, 1996, 35 (21): : 4117 - 4123
  • [18] Instrument response standard in time-resolved fluorescence
    Luchowski, Rafal
    Gryczynski, Zygmunt
    Sarkar, Pabak
    Borejdo, Julian
    Szabelski, Mariusz
    Kapusta, Peter
    Gryczynski, Ignacy
    REVIEW OF SCIENTIFIC INSTRUMENTS, 2009, 80 (03)
  • [19] Introduction to Time-Resolved Spectroscopy: Nanosecond Transient Absorption and Time-Resolved Fluorescence of Eosin B
    Farr, Erik P.
    Quintana, Jason C.
    Reynoso, Vanessa
    Ruberry, Josiah D.
    Shin, Wook R.
    Swartz, Kevin R.
    JOURNAL OF CHEMICAL EDUCATION, 2018, 95 (05) : 864 - 871
  • [20] Miniaturized Time-Resolved Fluorescence Spectroscopy System
    Gasser, Anas
    Shcheglov, Artem
    Lapauw, Thomas
    van den Dries, Thomas
    Ingelberts, Hans
    Hoving, Willem
    Aalders, Maurice
    Kuijk, Maarten
    PHOTONIC INSTRUMENTATION ENGINEERING IX, 2022, 12008
12345