A novel recombinant serotype 4 fowl adenovirus expressing fiber-2 protein of duck adenovirus 3

被引：4
作者：
Guo, Yiwen [1 ,2 ,3 ,4 ]
Lin, Yun [1 ,2 ,3 ]
Xie, Quan [1 ,2 ,3 ,4 ]
Zhang, Wenyuan [1 ,2 ,3 ,4 ]
Xu, Zhenqi [1 ,2 ,3 ,4 ]
Chao, Yifei [1 ,2 ,3 ,4 ]
Cao, Xudong [1 ,2 ,3 ,4 ]
Jiang, Huiru [1 ,2 ,3 ,4 ]
Li, Han [1 ,2 ,3 ,4 ]
Li, Tuofan [1 ,2 ,3 ,4 ]
Wan, Zhimin [1 ,2 ,3 ,4 ]
Shao, Hongxia [1 ,2 ,3 ,4 ]
Qin, Aijian [1 ,2 ,3 ,4 ]
Ye, Jianqiang [1 ,2 ,3 ,4 ]
机构：
[1] Yangzhou Univ, Coll Vet Med, Key Lab Jiangsu Prevent Vet Med, Key Lab Avian Prevent Med,Minist Educ, Yangzhou, Jiangsu, Peoples R China
[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China
[3] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China
[4] Yangzhou Univ, Inst Agr Sci & Technol Dev, Yangzhou, Jiangsu, Peoples R China
来源：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2023年 / 13卷
关键词：
DAdV-3; fiber-2; recombinant FAdV-4; CRISPR; Cas9; expression; replication; VACCINE;
D O I：
10.3389/fcimb.2023.1177866
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Recently, the highly pathogenic serotype 4 fowl adenovirus (FAdV-4) and duck adenovirus 3 (DAdV-3) were outbroken and widespread, causing substantial economic losses to the duck industry. Therefore, there is an urgent need to generate a recombinant genetic engineering vaccine candidate against both FAdV-4 and DAdV-3. In this study, a novel recombinant FAdV-4 expressing the Fiber-2 protein of DAdV-3, designated as rFAdV-4-Fiber-2/DAdV-3, was generated based on CRISPR/Cas9 and Cre-LoxP systems. Indirect immunofluorescence assay (IFA) and western blot (WB) showed that the Fiber-2 protein of DAdV-3 in rFAdV-4-Fiber-2/DAdV-3 was expressed successfully. Moreover, the growth curve revealed that rFAdV-4-Fiber-2/DAdV-3 replicated efficiently in LMH cells and even showed a stronger replication ability compared to the wild type FAdV-4. The generation of the recombinant rFAdV-4-Fiber-2/DAdV-3 provides a potential vaccine candidate against both FAdV-4 and DAdV-3.
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页数：7
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