Bioremediation of phenanthrene in saline-alkali soil by biochar- immobilized moderately halophilic bacteria combined with Suaeda salsa L.

Polycyclic aromatic hydrocarbon (PAH) contaminated saline-alkali soil is commonly salinized and hardened, which leads to low self-purification efficiency, making it difficult to reuse and remediate. In this study, pot experiments were conducted to investigate remediation of PAH contaminated saline-alkali soil using biochar-immobilized Martelella sp. AD-3, and Suaeda salsa L (S. salsa). Reduction in phenanthrene concentration, PAH degradation func-tional genes, and the microbial community in the soil were analyzed. The soil properties and plant growth parameters were also analyzed. After a 40-day remediation, the removal rate of phenanthrene by biochar-immobilized bacteria combined with S. salsa (MBP group) was 91.67 %. Additionally, soil pH and electrical conductivity (EC) reduced by 0.15 and 1.78 ds/m, respectively. The fresh weight and leaf pigment contents increased by 1.30 and 1.35 times, respec-tively, which effectively alleviated the growth pressure on S. salsa in PAH-contaminated saline-alkali soil. Further-more, this remediation resulted in abundance of PAH degradation functional genes in the soil, with a value of 2.01 x 103 copies/g. The abundance of other PAH degraders such as Halomonas, Marinobacter, and Methylophaga in soil also increased. Furthermore, the highest abundance of Martelella genus was observed after the MBP treatment, in-dicating that strain AD-3 has a higher survival ability in the rhizosphere of S. salsa under the protection of biochar. This study provides a green, low-cost technique for remediation of PAH-contaminated saline-alkali soils.