Redox Regulation of Salt Tolerance in Eutrema salsugineum by Proteomics

被引:2
|
作者
Li, Jiawen [1 ]
Yang, Xiaomin [1 ]
Liu, Fuqing [1 ]
Liu, Xinxin [1 ]
Zhao, Tong [1 ]
Yan, Xiufeng [2 ]
Pang, Qiuying [1 ]
机构
[1] Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China
[2] Wenzhou Univ, Coll Life & Environm Sci, Zhejiang Prov Key Lab Water Environm & Marine Biol, Wenzhou 325035, Peoples R China
基金
中国国家自然科学基金;
关键词
Eutrema salsugineum; NaCl; TMT; redox proteomics; glutathione peroxidase; GLUTATHIONE-PEROXIDASE; PLANT; STRESS; GENE; IDENTIFICATION; THELLUNGIELLA; MECHANISMS; EXPRESSION; INSIGHTS; GENOME;
D O I
10.3390/ijms241914518
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salt stress severely restricts plant growth and crop production, which is accompanied by accumulation of reactive oxygen species (ROS) that disturb cell redox homeostasis and oxidize redox-sensitive proteins. Eutrema salsugineum, a halophytic species closely related to Arabidopsis, shows a high level of tolerance to salinity and is increasingly used as a model plant in abiotic stress biology. To understand redox modifications and signaling pathways under salt stress, we used tandem mass tag (TMT)-based proteomics to quantify the salt-induced changes in protein redox modifications in E. salsugineum. Salt stress led to increased oxidative modification levels of 159 cysteine sites in 107 proteins, which play roles in carbohydrate and energy metabolism, transport, ROS homeostasis, cellular structure modulation, and folding and assembly. These lists of unknown redox reactive proteins in salt mustard lay the foundation for future research to understand the molecular mechanism of plant salt response. However, glutathione peroxidase (GPX) is one of the most important antioxidant enzymes in plants. Our research indicates that EsGPX may be involved in regulating ROS levels and that plants with overexpressed EsGPX have much improved salt tolerance.
引用
收藏
页数:15
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