LncRNA FGD5-AS1 Promotes Abdominal Aortic Aneurysm Growth Through the Activation of MMP3 in Vascular Smooth Muscle Cells

被引：3

作者：

Xu, Zhongjian ^{[1
,2
]}

Lang, Dehai ^{[1
,2
]}

Wang, Di ^{[1
,2
]}

Hu, Songjie ^{[1
,2
]}

Yang, Leibo ^{[1
,2
]}

机构：

[1] Univ Chinese Acad Sci, Hwa Mei Hosp, Dept Vasc Surg, 41 Xibei St, Ningbo 315000, Zhejiang, Peoples R China

[2] Ningbo 2 Hosp, Dept Vasc Surg, Ningbo, Zhejiang, Peoples R China

来源：

INTERNATIONAL HEART JOURNAL | 2023年 / 64卷 / 03期

关键词：

Downstream target protein; miRNA; Proliferation; Apoptosis; miR-195-5p; Ang-II perfusion model; MECHANISMS; MIGRATION;

D O I：

10.1536/ihj.22-106

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Long noncoding RNAs (lncRNAs) can serve as treatment targets for abdominal aortic aneurysms (AAAs). Nonetheless, the exact role of FGD5 antisense RNA 1 (FGD5-AS1) in AAAs is unclear. Therefore, this study investigated the contribution of FGD5-AS1 to AAA growth regulated by vascular smooth muscle cells (VSMCs) and its potential mechanisms. ApoE-/- mice were used to establish the angiotensin II (Ang II)-elicited AAA model. RNA pull-down assay and dual luciferase reporter assay (DLRA) in human VSMCs were used in exam-ining the interactions between FGD5-AS1 and its downstream proteins or miRNA targets. FGD5-AS1 expres-sion in the mouse Ang II perfusion group was dramatically increased relative to the PBS-infused group. In the mouse AAA model, FGD5-AS1 overexpression induced SMC apoptosis, thereby promoting AAA growth. miR-195-5p acts as a potential FGD5-AS1 downstream target, whereas FGD5-AS1 promotes MMP3 expression by inhibiting miR-195-5p expression, thereby inhibiting proliferation and promoting apoptosis of smooth muscle cells. LncRNA FGD5-AS1 is detrimental to the proliferation and survival of SMCs during AAA growth. There-fore, FGD5-AS1 could be a novel treatment target for AAA.

引用

页码：470 / 482

页数：13

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