Prevalence and Characterization of Staphylococcus aureus Isolated from Retail Raw Milk Samples in Chennai, India

被引:3
作者
Deepak, Shankaregowdanakopalu Jagadeesh [1 ]
Kannan, Porteen [1 ]
Savariraj, Wilfred Ruban [2 ]
Ghatak, Sandeep [3 ]
Ayyasamy, Elango [4 ,5 ]
Senthil Kumar, Tuticorin Maragatham Alagesan [6 ]
Ravindran, Narendra Babu [7 ]
Sundaram, Sureshkannan [1 ]
Kang, Qing [8 ]
Cull, Charley A. [9 ]
Amachawadi, Raghavendra G. [10 ]
机构
[1] Tamil Nadu Vet & Anim Sci Univ, Madras Vet Coll, Dept Vet Publ Hlth & Epidemiol, Chennai 600007, Tamil Nadu, India
[2] KVAFSU, Vet Coll, Dept Livestock Prod & Technol, Bengaluru, Karnataka, India
[3] ICAR Res Complex NEH Reg, Div Anim & Fisheries Sci, Umiam, Meghalaya, India
[4] Tamil Nadu Vet & Anim Sci Univ, Vet Coll, Salem, Tamil Nadu, India
[5] Tamil Nadu Vet & Anim Sci Univ, Res Inst, Salem, Tamil Nadu, India
[6] Tamil Nadu Vet & Anim Sci Univ, Ctr Anim Hlth Studies, Zoonoses Res Lab, Chennai, Tamil Nadu, India
[7] Tamil Nadu Vet & Anim Sci Univ, Madras Vet Coll, Dept Livestock Prod & Technol, Chennai, Tamil Nadu, India
[8] Kansas State Univ, Dept Stat, Manhattan, KS USA
[9] Vet & Biomed Res Ctr Inc, Manhattan, KS USA
[10] Kansas State Univ, Coll Vet Med, Dept Clin Sci, Manhattan, KS 66506 USA
关键词
antimicrobial resistance; foods of animal origin; pulsed-field gel electrophoresis; genetic diversity; FIELD GEL-ELECTROPHORESIS; RESISTANCE; FOOD; PRODUCTS; MRSA;
D O I
10.1089/fpd.2023.0050
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Milk is an important source of food, and it is also a nutrient-rich medium, which can harbor multiple microorganisms. Staphylococcus aureus is an important foodborne pathogen in food-producing animals, and there have been many reports on its infection and antimicrobial resistance (AMR), which has significant global public health concerns. This study was designed to isolate, characterize, and analyze the AMR pattern of S. aureus from milk samples collected in Chennai, India. A total of 259 raw milk samples from 3 groups: dairy farms, local vendors, and retail outlets were analyzed, and it was found that 34% (89/259) were positive for S. aureus. Positive isolates were further characterized by pulsed-field gel electrophoresis and isolates recovered from different sources, study areas, and locations showed high genetic diversity with no similarity. The presence of AMR has been further assessed by phenotypic methods as per CLSI-M100 performance standards, and all the isolates were susceptible to ampicillin/sulbactam, mupirocin, and tylosin. Additionally, all of the isolates were resistant to ampicillin. There were 28 isolates categorized as multidrug-resistant, which showed resistance to more than 2-3 classes of antimicrobials. This is the first report of inducible clindamycin resistance and mupirocin sensitivity pattern from S. aureus isolates recovered from milk. This study established the occurrence varied with genetic diversity in the isolates prevalent in the study area and divergence pattern of AMR S. aureus. The AMR in these isolates and with methicillin-resistant S. aureus could pose a serious threat to food safety and economic implications.
引用
收藏
页码:570 / 578
页数:9
相关论文
共 50 条
[31]   Molecular Typing and Antimicrobial Susceptibility of Staphylococcus aureus Strains Isolated from Raw Milk, Cheese, Minced Meat, and Chicken Meat Samples [J].
Can, Hayriye Yesim ;
Elmali, Mehmet ;
Karagoz, Alper .
KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES, 2017, 37 (02) :175-180
[32]   Enterotoxigenicity and Antimicrobial Resistance of Staphylococcus aureus Isolated from Retail Food in China [J].
Wang, Wei ;
Baloch, Zulqarnain ;
Jiang, Tao ;
Zhang, Cunshan ;
Peng, Zixin ;
Li, Fengqin ;
Fanning, Seamus ;
Ma, Aiguo ;
Xu, Jin .
FRONTIERS IN MICROBIOLOGY, 2017, 8
[33]   Prevalence and molecular characterization of Staphylococcus aureus isolated from goats in Chongqing, China [J].
Zhou, Zuoyong ;
Zhang, Mengsi ;
Li, Hexian ;
Yang, Haoyue ;
Li, Xiaoxia ;
Song, Xinyue ;
Wang, Zhiying .
BMC VETERINARY RESEARCH, 2017, 13
[34]   The prevention and removal of biofilm formation of Staphylococcus aureus strains isolated from raw milk samples by citric acid treatments [J].
Akbas, Meltem Yesilcimen ;
Kokumer, Tugba .
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2015, 50 (07) :1666-1672
[35]   Prevalence, Antimicrobial Resistance, and Molecular Characteristics of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Ice Cream in Shaanxi Province, China [J].
Zhang, Pengfei ;
Liu, Xinyu ;
Zhang, Meng ;
Kou, Mingying ;
Chang, Guanhong ;
Wan, Yangli ;
Xu, Xu ;
Ruan, Fuqian ;
Wang, Yeru ;
Wang, Xin .
FOODBORNE PATHOGENS AND DISEASE, 2022, 19 (03) :217-225
[36]   Prevalence, antibiotic resistance, and enterotoxin production of Staphylococcus aureus isolated from retail raw beef, sheep, and lamb meat in Turkey [J].
Sanlibaba, Pinar .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2022, 361
[37]   Characterization of Staphylococcus aureus isolates from raw milk sources in Victoria, Australia [J].
McMillan, Kate ;
Moore, Sean C. ;
McAuley, Catherine M. ;
Fegan, Narelle ;
Fox, Edward M. .
BMC MICROBIOLOGY, 2016, 16
[38]   Diversity of Staphylococcus aureus enterotoxin types within single samples of raw milk and raw milk products [J].
Loncarevic, S ;
Jorgensen, HJ ;
Lovseth, A ;
Mathisen, T ;
Rorvik, LM .
JOURNAL OF APPLIED MICROBIOLOGY, 2005, 98 (02) :344-350
[39]   Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java']Java, Indonesia [J].
Tyasningsih, Wiwiek ;
Ramandinianto, Sancaka Chasyer ;
Ansharieta, Ribby ;
Witaningrum, Adiana Mutamsari ;
Permatasari, Dian Ayu ;
Wardhana, Dhandy Koesoemo ;
Effendi, Mustofa Helmi ;
Ugbo, Emmanuel Nnabuike .
VETERINARY WORLD, 2022, 15 (08) :2021-2028
[40]   Isolation and Characterization of Staphylococcus aureus in Bovine Milk from Rewa, India [J].
Rawat, Shivani ;
Shrivastava, Neeraj ;
Shrivastav, Arpita ;
Singh, Shailendra ;
Singh, P. K. ;
Niranjan, Ankush Kiran ;
Ranjan, Rajeev .
INDIAN JOURNAL OF MICROBIOLOGY, 2024, 64 (04) :1835-1845