Molecular and Cellular Mechanism of Action of Chrysotile Asbestos in MRC5 Cell Line

被引：0
作者：
Kussainova, Assiya [1 ,2 ]
Bulgakova, Olga [2 ]
Aripova, Akmaral [2 ]
Ibragimova, Milana [2 ,3 ]
Pulliero, Alessandra [1 ]
Begimbetova, Dinara [3 ]
Bersimbaev, Rakhmetkazhi [2 ]
Izzotti, Alberto [4 ,5 ]
机构：
[1] Univ Genoa, Dept Hlth Sci, Via Pastore 1, I-16132 Genoa, Italy
[2] LN Gumilyov Eurasian Natl Univ, Inst Cell Biol & Biotechnol, Dept Gen Biol & Genom, Astana 010008, Kazakhstan
[3] Nazarbayev Univ, Natl Lab Astana, Astana 010000, Kazakhstan
[4] Univ Genoa, Dept Expt Med, I-16132 Genoa, Italy
[5] IRCCS Osped Policlin San Martino, I-16132 Genoa, Italy
来源：
JOURNAL OF PERSONALIZED MEDICINE | 2023年 / 13卷 / 11期
关键词：
chrysotile asbestos; reactive oxygen species; cf mtDNA; microRNA; lung cancer; MITOCHONDRIAL-DNA; EPITHELIAL-CELLS; LUNG-CANCER; EXPOSURE; TRANSITION; APOPTOSIS; SERUM;
D O I：
10.3390/jpm13111599
中图分类号：
R19 [保健组织与事业（卫生事业管理）];
学科分类号：
摘要：
Asbestos is a known carcinogen; however, the influence of chrysotile asbestos on the development of tumor-related diseases remains a subject of intense debate within the scientific community. To analyze the effect of asbestos, we conducted a study using the MRC5 cell line. We were able to demonstrate that chrysotile asbestos stimulated the production of reactive oxygen species (ROS), leading to cell death and DNA damage in the MRC5 cell line, using various techniques such as ROS measurement, comet assay, MTT assay, and qPCR. In addition, we found that chrysotile asbestos treatment significantly increased extracellular mitochondrial DNA levels in the culture medium and induced significant changes in the expression profile of several miRNAs, which was the first of its kind. Thus, our research highlights the importance of studying the effects of chrysotile asbestos on human health and reveals multiple adverse effects of chrysotile asbestos.
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页数：16
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