Bioconversion of feather waste into bioactive nutrients in water by Bacillus licheniformis WHU

被引:5
作者
Zhang, Jing [1 ]
Liang, Manyu [1 ]
Wu, Lijuan [2 ]
Yang, Yanhong [1 ]
Sun, Yingjie [1 ]
Wang, Qin [1 ,3 ]
Gao, Xiaowei [1 ,3 ,4 ]
机构
[1] Southwest Med Univ, Sch Pharm, Luzhou 646000, Sichuan, Peoples R China
[2] Southwest Med Univ, Affiliated Tradit Chinese Med Hosp, Dept Endocrinol, Luzhou 646000, Sichuan, Peoples R China
[3] Dazhou Vocat Coll Chinese Med, Dazhou 635000, Peoples R China
[4] Southwest Med Univ, Green Pharmaceut Technol Key Lab Luzhou, Luzhou 646000, Sichuan, Peoples R China
关键词
Hydrolysis; Keratinase; Bacillus licheniformis; Gut microbiota; Peptide; Amino acids; Intestinal barrier; MICROBIAL KERATINASES; INDIGENOUS BACTERIA; CHICKEN FEATHERS; SOY-PROTEIN; ANTIOXIDANT; FERMENTATION; DEGRADATION; PERFORMANCE; EXPRESSION; INCLUSION;
D O I
10.1007/s00253-023-12795-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Feathers become hazardous pollutants when deposited directly into the environment. The rapid expansion of the poultry industry has significantly increased feather waste, necessitating the development of new ways to degrade and utilize feathers. This study investigated the ability of Bacillus licheniformis WHU to digest intact chicken feathers in water. The results indicated that yields of free amino acids, bioactive peptides, and keratin-derived nano-/micro-particles were improved in bacteria- versus purified keratinase-derived feather hydrolysate. Bacteria-derived feather hydrolysate supplementation induced health benefits in mice, including significantly increased intestinal villus height and zonula occludens-1 protein expression, as well as increased secretory immunoglobulin A levels in the intestinal mucosa and superoxide dismutase activity in serum. Additionally, feather hydrolysate supplementation modulated the mouse gut microbiota, reflected by increased relative abundance of probiotics such as Lactobacillus spp., decreased relative abundance of Proteobacteria at the phylum level and pathogens such as Staphylococcus spp., and increased Bacteroidota/Firmicutes ratio. This study developed a simple, cost-effective method to degrade feathers by B. licheniformis WHU digestion, yielding a hydrolysate that can be directly used as a bioactive nutrient resource. The study findings have applications in the livestock, poultry, and aquaculture industries, which have high demands for cheap protein.
引用
收藏
页码:7055 / 7070
页数:16
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