Two-photon manipulation of neuronal activity and behavior in

被引：0
|
作者：
Yamamoto, Wataru [1 ]
Yuste, Rafael [1 ]
机构：
[1] Columbia Univ, NeuroTechnol Ctr, Dept Biol Sci, New York, NY 10027 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 03期
关键词：
HYDRA; MEDUSAE;
D O I：
10.1016/j.xpro.2023.102453
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.
引用
收藏
页数：14
相关论文
共 50 条
  • [41] Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain
    Stirman, Jeffrey N.
    Smith, Ikuko T.
    Kudenov, Michael W.
    Smith, Spencer L.
    NATURE BIOTECHNOLOGY, 2016, 34 (08) : 857 - +
  • [42] Two-photon imaging of neuronal activity in motor cortex of marmosets during upper-limb movement tasks
    Teppei Ebina
    Yoshito Masamizu
    Yasuhiro R. Tanaka
    Akiya Watakabe
    Reiko Hirakawa
    Yuka Hirayama
    Riichiro Hira
    Shin-Ichiro Terada
    Daisuke Koketsu
    Kazuo Hikosaka
    Hiroaki Mizukami
    Atsushi Nambu
    Erika Sasaki
    Tetsuo Yamamori
    Masanori Matsuzaki
    Nature Communications, 9
  • [43] Physics-Based Deep Learning for Imaging Neuronal Activity via Two-Photon and Light Field Microscopy
    Verinaz-Jadan, Herman
    Howe, Carmel L.
    Song, Pingfan
    Lesept, Flavie
    Kittler, Josef
    Foust, Amanda J.
    Dragotti, Pier Luigi
    IEEE TRANSACTIONS ON COMPUTATIONAL IMAGING, 2023, 9 : 565 - 580
  • [44] Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain
    Jeffrey N Stirman
    Ikuko T Smith
    Michael W Kudenov
    Spencer L Smith
    Nature Biotechnology, 2016, 34 : 857 - 862
  • [45] Population Level Profiling of Primary Afferent Neuronal Activity in Response to Epinephrine Using Two-Photon Calcium Imaging
    Stratton, Harrison J.
    Warwick, Charlies
    Koerber, H. Richard
    Ross, Sarah E.
    JOURNAL OF PAIN, 2024, 25 (04): : 19 - 20
  • [46] Two-photon microscopy
    Diaspro, A
    IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1999, 18 (05): : 16 - 17
  • [47] Two-photon beats
    Jeffers, J
    Barnett, SM
    JOURNAL OF MODERN OPTICS, 1996, 43 (10) : 1985 - 1992
  • [48] Two-photon physics
    M. Vanderhaeghen
    The European Physical Journal A - Hadrons and Nuclei, 2006, 28 : 71 - 80
  • [49] Two-photon lasers
    Gauthier, DJ
    PROGRESS IN OPTICS, VOL 45, 2003, 45 : 205 - 272
  • [50] Two-photon vision
    Graydon, Oliver
    NATURE PHOTONICS, 2018, 12 (01) : 2 - 2
12345