Two-photon manipulation of neuronal activity and behavior in

被引：0
|
作者：
Yamamoto, Wataru [1 ]
Yuste, Rafael [1 ]
机构：
[1] Columbia Univ, NeuroTechnol Ctr, Dept Biol Sci, New York, NY 10027 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 03期
关键词：
HYDRA; MEDUSAE;
D O I：
10.1016/j.xpro.2023.102453
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments.
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页数：14
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