Monitoring Melanoma Responses to STING Agonism and Focused Ultrasound Thermal Ablation Using Microneedles and Ultrasensitive Single Molecule Arrays

被引：7

作者：

Dahis, Daniel ^{[1
,2
,3
,4
]}

Dion, Michelle Z. ^{[2
,3
,4
,5
]}

Cryer, Alexander M. ^{[2
,3
,4
]}

Dosta, Pere ^{[2
,3
,4
]}

Gilboa, Tal ^{[4
,6
]}

Alonso, Mariana ^{[3
]}

Lewandowski, Michael ^{[4
]}

Puigmal, Nuria ^{[2
,3
,4
]}

Taboada, Gonzalo Munoz ^{[2
]}

Azhari, Haim ^{[1
]}

Ahmad, Rushdy ^{[4
]}

Walt, David R. ^{[4
,6
]}

Artzi, Natalie ^{[2
,3
,4
,7
]}

机构：

[1] Technion Israel Inst Technol, IL-320003 Haifa, Israel

[2] MIT, Inst Med Engn & Sci, Cambridge, MA 02139 USA

[3] Harvard Med Sch, Brigham & Womens Hosp, Boston, MA 02115 USA

[4] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[5] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[6] Harvard Med Sch Boston, Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA

[7] Broad Inst Harvard & MIT, Cambridge, MA 02139 USA

来源：

ADVANCED FUNCTIONAL MATERIALS | 2023年 / 33卷 / 50期

基金：

美国国家科学基金会;

关键词：

focused ultrasound thermal ablation; ISF biomarkers; melanoma; microneedles; nanoparticles; Simoa; STING; INTERSTITIAL FLUID; IMMUNO-PET; TUMOR; IMMUNOTHERAPY; SENSITIVITY; ACTIVATION; BIOMARKERS; INJURY; POTENT; ASSAY;

D O I：

10.1002/adfm.202301659

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Real-time monitoring of immune state and response to therapy can provide a means to stratify patients and increase the number of responders who will benefit from approved and emerging immunotherapies. The accessibility of immune cells in the skin provides an opportunity for local immune modulation as well as for noninvasive sampling of disease biomarkers in the skin interstitial fluid (ISF). Here, a monitoring strategy for melanoma immunotherapy is investigated by longitudinal sampling of biomarkers in the skin ISF using Hyaluronic acid (HA)-based microneedles (MNs). Focused ultrasound ablation and delivery of nanoparticulate stimulator of interferon genes agonist are used as model immunotherapies. It is shown that this combination therapy induces potent inflammatory responses in a melanoma mouse model, promoting tumor elimination and immune memory formation. Indeed, quantifying soluble, protein-based biomarkers following therapy using conventional immunoassay reveals a pronounced proinflammatory program in the tumor. However, conventional assays fail to detect the low concentration of biomarkers in plasma and in MN-sampled ISF. It is shown that ultrasensitive single molecule arrays (Simoa) effectively detected proinflammatory biomarkers that are upregulated in response to the therapy in MN-sampled ISF, in plasma, and in tumors, supporting the feasibility of monitoring melanoma immunotherapy using MNs.

引用

页数：13