Isovitexin prevents DSS-induced colitis through inhibiting inflammation and preserving intestinal barrier integrity through activating AhR

被引：12

作者：

Mu, Jianfeng ^{[1
]}

Song, Jiaxing ^{[2
]}

Li, Rong ^{[3
]}

Xue, Tianyi ^{[3
]}

Wang, Dongxu ^{[3
]}

Yu, Jinhai ^{[1
]}

机构：

[1] First Hosp Jilin Univ, Gen Surg Ctr, Dept Gastr & Colorectal Surg, Changchun, Jilin, Peoples R China

[2] First Hosp Jilin Univ, Dept Lab Med, Changchun, Peoples R China

[3] Jilin Univ, Coll Anim Sci, Lab Anim Ctr, Changchun, Peoples R China

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2023年 / 382卷

关键词：

Isovitexin; Colitis; DSS; AhR; NF-KAPPA-B; BOWEL-DISEASE; EPIDEMIOLOGY; MANAGEMENT; INJURY; CELLS;

D O I：

10.1016/j.cbi.2023.110583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isovitexin (ISO) is a glycosylated flavonoid obtained from Asian rice that has been reported to have antiinflammatory effect. However, the effects of ISO on colitis have not been reported. In the present study, we aimed to explore the protective effects of isovitexin on colitis using the dextran sodium sulfate (DSS)-induced model. In vitro, the protective mechanism was investigated in TNF-& alpha;-stimulated IEC cells. Inflammatory cytokines were measured by ELISA. The signaling pathways were measured by Western blot analysis. ISO attenuated DSS-induced colitis through reducing body weight loss and colonic histological changes. Also, the levels of TNF-& alpha; and IL-1 & beta; induced by DSS were inhibited by ISO. The MPO activity induced by DSS was attenuated by ISO. In vitro, ISO inhibited IL-6 and IL-1 & beta; production in TNF-& alpha;-stimulated cells. ISO increased the expression of tight junction proteins ZO-1 and occludin. Also, ISO inhibited TNF-& alpha;-induced NF-& kappa;B activation. In addition, ISO was found to increase the expression of aryl hydrocarbon receptor (AhR). And inhibition of AhR by its antagonist CH223191 could reverse these effects of ISO. ISO inhibited DSS-induced colitis in mice through suppressing inflammation and preserving intestinal barrier integrity through activating AhR. ISO may be useful as a potential therapeutic agent for colitis.

引用

页数：8

共 23 条

[1] The role of nuclear factor-kappa B in cytokine gene regulation [J].

Blackwell, TS ;

Christman, JW .

AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1997, 17 (01) :3-9

[2] The immunological and genetic basis of inflammatory bowel disease [J].

Bouma, G ;

Strober, W .

NATURE REVIEWS IMMUNOLOGY, 2003, 3 (07) :521-533

[3] Epidemiology and Natural History of Inflammatory Bowel Diseases [J].

Cosnes, Jacques ;

Gower-Rousseau, Corinne ;

Seksik, Philippe ;

Cortot, Antoine .

GASTROENTEROLOGY, 2011, 140 (06) :1785-U118

[4] The glycosylated flavonoids vitexin, isovitexin, and quercetrin isolated from Serjania erecta Radlk (Sapindaceae) leaves protect PC12 cells against amyloid-β25-35 peptide-induced toxicity [J].

Guimaraes, Camila Carla ;

Oliveira, Denise Dias ;

Valdevite, Mayara ;

Fachin Saltoratto, Ana Lucia ;

Vaz Pereira, Sarazete Izidia ;

Franca, Suzelei de Castro ;

Soares Pereira, Ana Maria ;

Pereira, Paulo Sergio .

FOOD AND CHEMICAL TOXICOLOGY, 2015, 86 :88-94

[5] Impact of hospital volume on postoperative morbidity and mortality following a colectomy for ulcerative colitis [J].

Kaplan, Gilaad G. ;

Mccarthy, Ellen P. ;

Ayanian, John Z. ;

Korzenik, Joshua ;

Hodin, Richard ;

Sands, Bruce E. .

GASTROENTEROLOGY, 2008, 134 (03) :680-687

[6] Melatonin reduces inflammatory injury through inhibiting NF-κB activation in rats with colitis [J].

Li, JH ;

Yu, JP ;

Yu, HG ;

Xu, XM ;

Yu, LL ;

Liu, J ;

Luo, HS .

MEDIATORS OF INFLAMMATION, 2005, (04) :185-193

[7] Novel Pentapeptide Derived from Chicken by-Product Ameliorates DSS-Induced Colitis by Enhancing Intestinal Barrier Function via AhR-Induced Src Inactivation [J].

Li, Shanshan ;

Ma, Bin ;

Wang, Jin ;

Peng, Hengying ;

Zheng, Meng ;

Dai, Wenling ;

Liu, Jihua .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2020, 68 (48) :14192-14203

[8] Isovitexin suppresses lipopolysaccharide-mediated inducible nitric oxide synthase through inhibition of NF-kappa B in mouse macrophages [J].

Lin, CM ;

Huang, ST ;

Liang, YC ;

Lin, MS ;

Shih, CM ;

Chang, YC ;

Chen, TY ;

Chen, CT .

PLANTA MEDICA, 2005, 71 (08) :748-753

[9] Baicalein Restores the Balance of Th17/Treg Cells via Aryl Hydrocarbon Receptor to Attenuate Colitis [J].

Liu, Chang ;

Li, Yanyang ;

Chen, Yanping ;

Huang, Shaowei ;

Wang, Xiaojing ;

Luo, Shuang ;

Su, Yulin ;

Zhou, Lian ;

Luo, Xia .

MEDIATORS OF INFLAMMATION, 2020, 2020

[10] Isovitexin Exerts Anti-Inflammatory and Anti-Oxidant Activities on Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting MAPK and NF-κB and Activating HO-1/Nrf2 Pathways [J].

Lv, Hongming ;

Yu, Zhenxiang ;

Zheng, Yuwei ;

Wang, Lidong ;

Qin, Xiaofeng ;

Cheng, Genhong ;

Ci, Xinxin .

INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2016, 12 (01) :72-86

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