Subcellular distribution of membrane lipids revealed by freeze-fracture electron microscopy

被引：0
作者：
Tsuji, Takuma [1 ]
机构：
[1] Juntendo Univ, Res Inst Dis Old Age, Lab Mol Cell Biol, Grad Sch Med, Tokyo 1138421, Japan
来源：
ANATOMICAL SCIENCE INTERNATIONAL | 2024年 / 99卷 / 01期
关键词：
Electron microscopy; Lipid bilayer; Membrane lipid; Organelle membrane; Scramblase; PHOSPHATIDYLSERINE; SCRAMBLASE; DEPTH;
D O I：
10.1007/s12565-023-00731-9
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Cell membranes are composed of a large variety of lipids and proteins. While the localization and function of membrane proteins have been extensively investigated, the distribution of membrane lipids, especially in the non-cytoplasmic leaflet of organelle membranes, remains largely unknown. Fluorescent biosensors have been widely used to study membrane lipid distribution; however, they have some limitations. By utilizing the quick-freezing and freeze-fracture replica labeling electron microscopy technique, we can uncover the precise distribution of membrane lipids within cells and assess the function of lipid-transporting proteins. In this review, I summarize recent progress in analyzing intracellular lipid distribution by utilizing this method.
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页码：1 / 6
页数：6
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