The hypothalamic neuropeptide, QRFP, regulates high fat diet intake in female Long-Evans rats following ovariectomy

被引:0
|
作者
Martin, Cade T. [1 ]
Primeaux, Stefany D. [1 ,2 ,3 ]
机构
[1] LSU Hlth Sci Ctr NO, Dept Physiol, New Orleans, LA 70112 USA
[2] Pennington Biomed Res Ctr, Joint Diabet Endocrinol & Metab Program, Baton Rouge, LA 70808 USA
[3] LSU Hlth NO, Dept Physiol, 1901 Perdido St, New Orleans, LA 70112 USA
基金
美国国家卫生研究院;
关键词
QRFP; Estradiol; High fat diet; Mediobasal hypothalamus; SiRNA; CENTRAL-NERVOUS-SYSTEM; MESSENGER-RNA EXPRESSION; FOOD-INTAKE; GENE-EXPRESSION; BODY-WEIGHT; ESTRADIOL; BEHAVIOR; OBESITY; NEURONS; HEALTH;
D O I
10.1016/j.peptides.2023.170960
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Obesity rates in women continue to increase throughout the lifespan and obesity-related comorbidities are prevalent in women in estrogen deficiency. The hypothalamic neuropeptide, QRFP, is an orexigenic peptide that increases the intake of high fat diet (HFD) in female rats and is overexpressed following ovariectomy (OVX). Therefore, the goal of the current series of experiments was to elucidate the effect of QRFP on HFD intake following OVX and determine if QRFP-26 administration in ovariectomized females altered expression of prepro-neuropeptide Y (NPY), agouti-related peptide (AgRP) and proopiomelanocortin (POMC) mRNA in the mediobasal hypothalamus (MBH) and prepro-orexin in the lateral hypothalamus (LH). The intake of HFD was measured following acute administration of QRFP-26 prior to or following estradiol benzoate (EB) treatment in ovariectomized females. When administered prior to EB treatment, QRFP-26 increased HFD intake. EB treatment attenuated the effects of QRFP-26 on HFD intake. Sub-chronic, continuous administration of QRFP-26 increased HFD intake and weight gain following OVX. Subchronic, continuous administration of QRFP siRNA into the 3rd ventricle via osmotic pump decreased prepro-QRFP mRNA levels in the MBH by similar to 75%, decreased HFD intake and decreased weight gain following OVX. QRFP-26administration did not alter the expression of prepro-NPY, AgRP or POMC mRNA in the MBH, but decreased prepro-orexin mRNA in the LH of ovariectomized females. Overall, results from these studies support the orexigenic neuropeptide, QRFP, as an important mediator of the ingestion of highly palatable foods and subsequent weight gain in females during estrogen deficiency.
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页数:9
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