Stable expression and multi-site location of Odf2 in mouse oocytes, sperm and early embryos

被引：1

作者：

Xu, Xiangning ^{[2
]}

Jiang, Xiuying ^{[1
]}

Song, Ke ^{[2
]}

Zhang, Jiaqi ^{[2
]}

Tian, Ying ^{[2
]}

Chen, Ye ^{[2
]}

Weng, Jing ^{[2
]}

Liang, Yuanjing ^{[2
]}

Ma, Wei ^{[2
,3
]}

机构：

[1] Beijing Sport Univ, Sch Sport Sci, Div Sport Anat, Beijing 100084, Peoples R China

[2] Capital Med Univ, Sch Basic Med Sci, Dept Histol & Embryol, Beijing 100069, Peoples R China

[3] Capital Med Univ, Sch Basic Med Sci, Dept Histol & Embryol, 10 XiTouTiao, Beijing 100069, Peoples R China

来源：

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER | 2023年 / 250卷

基金：

中国国家自然科学基金;

关键词：

Odf2; Oocyte; Spindle; MTOC; Vesicle; Sperm; Embryo; SPINDLE; CENTROSOME; CELLS;

D O I：

10.1016/j.aanat.2023.152126

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

In mammals, centriole is degenerated during early oogenesis, but it is still not known about the expression and function of centriolar structural components in oocyte meiosis. Here we found that Odf2 (outer dense fiber of sperm tails 2), a key centriolar appendage protein, was stably expressed in mouse oocytes during meiotic progression. Distinct from its single location at centrosomes in somatic mitosis, Odf2 was multiply located at microtubule organizing centers (MTOCs), chromosome centromeres and vesicles in oocyte meiosis. In addition, the vesicle-associated Odf2 disappeared in oocytes treated with the vesicle inhibitor Brefeldin A. Odf2 was mainly co-localized with the mitochondrial sheath in the sperm tail and presented as double spots, similar to & gamma;-tubulin, in the sperm neck region. After fertilization, Odf2 remained on vesicles in embryos from 1-cell to 4-cell stage but was only detected on centrosomes at blastocyst stage. Taken together, Odf2 is expressed precisely in mouse oocytes even in the absence of intact centriole structure, and may regulate oocyte spindle assembly and positioning, additionally, the sperm motility and early embryo development.

引用

页数：11