Microencapsulation of Bacillus subtilis and oat β-glucan and their application as a synbiotic in fish feed

To improve survival during storage and exposure to adverse conditions, Bacillus subtilis was microencapsulated with oat beta-glucan by spray-drying technology. The characterisation of the microcapsules was designed to compare free and microencapsulated cells through exposure to simulated gastric fluids (SGF) throughout storage for 90 days at different temperatures. The characterisation included analysis of efficiency, morphology, moisture, water activity, hygroscopicity, particle size, and zeta potential. The microcapsules presented a particle size of 1.5 +/- 0.34 mu m and an encapsulation efficiency of 77.9 +/- 3.06%. After SGF, the survival of microencapsulated cells was 8.4 +/- 0.07 log CFU mL(-1) while that of free cells was 7.6 +/- 0.06 log CFU mL(-1). After 90 days of storage, only microencapsulated cells remained above 6 log-unit of viability. In conclusion, spray-drying technique combined with the addition of oat beta-glucan proved to be an efficient method to protect B. subtilis under storage and SGF with potential application in fish feed.