Long non-coding RNA MRPL23-AS1 suppresses anoikis in salivary adenoid cystic carcinoma in vitro

被引：11
作者：
Li, Yin-Ran [1 ,2 ,3 ,4 ,5 ,6 ]
Fu, Min [1 ,2 ,3 ,4 ,5 ,6 ]
Song, Ye-Qing [1 ,2 ,3 ,4 ,5 ]
Li, Sheng-Lin [1 ,2 ,3 ,4 ,5 ,6 ]
Ge, Xi-Yuan [1 ,2 ,3 ,4 ,5 ,6 ]
机构：
[1] Peking Univ, Cent Lab, Sch & Hosp Stomatol, Beijing, Peoples R China
[2] Natl Ctr Stomatol, Beijing, Peoples R China
[3] Natl Clin Res Ctr Oral Dis, Natl Engn Res Ctr Oral Biomat & Digital Med Devic, Beijing, Peoples R China
[4] Minist Hlth, Res Ctr Engn & Technol Computerized Dent, Beijing Key Lab Digital Stomatol, Beijing, Peoples R China
[5] NMPA Key Lab Dent Mat, Beijing, Peoples R China
[6] Peking Univ, Dept Oral & Maxillofacial Surg, Sch & Hosp Stomatol, Beijing, Peoples R China
来源：
ORAL DISEASES | 2023年 / 29卷 / 04期
基金：
北京市自然科学基金; 中国国家自然科学基金;
关键词：
anoikis; LncRNA; MRPL23-AS1; p19INK4D; salivary adenoid cystic carcinoma; DISTANT METASTASES; MESENCHYMAL TRANSITION; HEAD; NECK; INHIBITORS; CELLS; DIFFERENTIATION; GALECTIN-3; APOPTOSIS; P19INK4D;
D O I：
10.1111/odi.14156
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Distant lung metastasis is the main factor that affects the survival rate of patients with salivary adenoid cystic carcinoma (SACC). Anoikis resistance is a feature of tumor cells that easily metastasize. The long non-coding RNA (lncRNA) MRPL23 antisense RNA 1 (MPRL23-AS1) is related to lung metastasis in SACC, but its role in anoikis resistance is unknown. After altering MPRL23-AS1 expression in SACC cells, anoikis resistance was detected by calcein AM/PI staining and annexin V/PI flow cytometry. The apoptosis marker activated caspase-3 and the bcl-2/bax ratio were detected by Western blotting. The relationship between MPRL23-AS1 and the promoter of the potential downstream target gene p19INK4D was identified by chromatin immunoprecipitation (ChIP)-PCR assay. p19INK4D expression in patient tissues was determined using qRT-PCR and immunohistochemistry. The functional experiments showed that MPRL23-AS1 could promote anoikis resistance in vitro. MRPL23-AS1 recruited the EZH2 to the promoter region of p19INK4D, inhibited p19INK4D expression, and promoted tumor cell anoikis resistance. p19INK4D overexpression did not affect anoikis in attached cells; however, it attenuated the anoikis resistance effect of MPRL23-AS1 in suspension cells. p19INK4D expression was significantly lower in SACC tissues than in normal tissues. The novel MRPL23-AS1/p19INK4D axis may be a potential SACC biomarker or therapeutic target.
引用
收藏
页码：1588 / 1601
页数：14
相关论文
共 56 条
[1]   CDK inhibitors: Cell cycle regulators and beyond [J].
Besson, Arnaud ;
Dowdy, Steven F. ;
Roberts, James M. .
DEVELOPMENTAL CELL, 2008, 14 (02) :159-169
[2]   E2F1 induces p19INK4d, a protein involved in the DNA damage response, following UV irradiation [J].
Carcagno, Abel L. ;
Giono, Luciana E. ;
marazita, Mariela C. ;
Castillo, Daniela S. ;
Pregi, Nicolas ;
Canepa, Eduardo T. .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2012, 366 (1-2) :123-129
[3]   Long Noncoding RNA MRPL23-AS1 Promoteoid Cystic Carcinoma Lung Metastasis [J].
Chen, Chu-Wen ;
Fu, Min ;
Du, Zhi-Hao ;
Zhao, Fei ;
Yang, Wen-Wen ;
Xu, Li-Hua ;
Li, Sheng-Lin ;
Ge, Xi-Yuan .
CANCER RESEARCH, 2020, 80 (11) :2273-2285
[4]   Investigation of myoepithelial cell differentiation into Schwann-like cells in salivary adenoid cystic carcinoma associated with perineural invasion [J].
Chen, Wei ;
Dong, Shaozhong ;
Zhou, Jun ;
Sun, Moyi .
MOLECULAR MEDICINE REPORTS, 2012, 6 (04) :755-759
[5]   Cell cycle inhibitors in normal and tumor stem cells [J].
Cheng, T .
ONCOGENE, 2004, 23 (43) :7256-7266
[6]   Adenoid cystic carcinoma of the head and neck - An update [J].
Coca-Pelaz, Andres ;
Rodrigo, Juan P. ;
Bradley, Patrick J. ;
Poorten, Vincent Vander ;
Triantafyllou, Asterios ;
Hunt, Jennifer L. ;
Strojan, Primoz ;
Rinaldo, Alessandra ;
Haigentz, Missak, Jr. ;
Takes, Robert P. ;
Mondin, Vanni ;
Teymoortash, Afshin ;
Thompson, Lester D. R. ;
Ferlito, Alfio .
ORAL ONCOLOGY, 2015, 51 (07) :652-661
[7]   LncRNA HOTAIR regulates anoikis-resistance capacity and spheroid formation of ovarian cancer cells by recruiting EZH2 and influencing H3K27 methylation [J].
Dai, Zhuo-Ya ;
Jin, Shuang-Mei ;
Luo, Hong-Qin ;
Leng, Hong-Lian ;
Fang, Jun-Dan .
NEOPLASMA, 2021, 68 (03) :509-518
[8]   The role of galectin-3 in the tumorigenesis and progression of pituitary tumors [J].
Diao, Bo ;
Liu, Ying ;
Xu, Guo-Zheng ;
Zhang, Yi ;
Xie, Jun ;
Gong, Jie .
ONCOLOGY LETTERS, 2018, 15 (04) :4919-4925
[9]   TGF-β1 Promotes Migration and Invasion of Salivary Adenoid Cystic Carcinoma [J].
Dong, L. ;
Wang, Y. X. ;
Li, S. L. ;
Yu, G. Y. ;
Gan, Y. H. ;
Li, D. ;
Wang, C. Y. .
JOURNAL OF DENTAL RESEARCH, 2011, 90 (06) :804-809
[10]   p19-INK4d inhibits neuroblastoma cell growth, induces differentiation and is hypermethylated and downregulated in MYCN-amplified neuroblastomas [J].
Dreidax, Daniel ;
Bannert, Steffen ;
Henrich, Kai-Oliver ;
Schroeder, Christina ;
Bender, Sebastian ;
Oakes, Christopher C. ;
Lindner, Sven ;
Schulte, Johannes H. ;
Duffy, David ;
Schwarzl, Thomas ;
Saadati, Maral ;
Ehemann, Volker ;
Benner, Axel ;
Pfister, Stefan ;
Fischer, Matthias ;
Westermann, Frank .
HUMAN MOLECULAR GENETICS, 2014, 23 (25) :6826-6837
123456