Serum CRP biomarker detection by using carbon nanotube field-effect transistor (CNT-FET) immunosensor

被引:35
作者
Rabbani, Gulam [1 ]
Khan, Mohammad Ehtisham [2 ]
Ahmad, Ejaz [3 ]
Khan, Mohsin Vahid [4 ]
Ahmad, Abrar [5 ]
Khan, Anwar Ulla [6 ]
Ali, Wahid [2 ]
Zamzami, Mazin A. [5 ]
Bashiri, Abdullateef H. [7 ]
Zakri, Waleed [7 ]
机构
[1] IT Med Fus Ctr, 350-27 Gumidae Ro, Gumi Si 35027, Gyeongbuk, South Korea
[2] Jazan Univ, Coll Appl Ind Technol, Dept Chem Engn Technol, Jazan 45142, Saudi Arabia
[3] Univ Michigan, Dept Pathol, Med Sch, Ann Arbor, MI 48109 USA
[4] Integral Univ, Dept Biosci, Lucknow 226026, India
[5] King Abdulaziz Univ, Fac Sci, Dept Biochem, Jeddah 21452, Saudi Arabia
[6] Jazan Univ, Coll Appl Ind Technol, Dept Elect Engn Technol, Jazan 45142, Saudi Arabia
[7] Jazan Univ, Coll Engn, Dept Mech Engn, POB 114, Jazan 45142, Saudi Arabia
关键词
Antigen-antibody; Carbon nanotubes; C-reactive protein; Field-effect transistor; Immunosensor; C-REACTIVE PROTEIN; CARDIOVASCULAR-DISEASE; RAMAN-SPECTROSCOPY; IONIC-STRENGTH; MARKERS; INFLAMMATION; ASSOCIATION; PREDICTION; CARE; PH;
D O I
10.1016/j.bioelechem.2023.108493
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
C-reactive protein (CRP) is produced by the liver in response to systemic inflammation caused by bacterial infection, trauma and internal organ failures. CRP serves as a potential biomarker in the precise diagnosis of cardiovascular risk, type-2 diabetes, metabolic syndrome, hypertension and various types of cancers. The pathogenic conditions indicated above are diagnosed by an elevated CRP level in the serum. In this study, we successfully fabricated a highly sensitive and selective carbon nanotube field-effect transistor (CNT-FET) immunosensor for the detection of CRP. The CNTs were deposited on the Si/SiO2 surface, between source-drain electrodes, afterwards modified with well-known linker PBASE and then anti-CRP was immobilized. This antiCRP functionalized CNT-FET immunosensor exhibits a wide dynamic detection range (0.01-1000 & mu;g/mL) CRP detection, rapid response time (2-3 min) and low variation (<3 %) which can be delivered as a low-cost and rapid clinical detection technology for the early diagnosis of coronary heart disease (CHD). For the clinical applications, our sensor was tested using CRP fortified serum samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). This CNT-FET immunosensor will be helpful in taking over the complex laboratory-based expensive traditional CRP diagnostic procedures practiced in the hospitals.
引用
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页数:11
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