Exosomes secreted by endothelial cells derived from human induced pluripotent stem cells improve recovery from myocardial infarction in mice

被引:17
|
作者
Li, Hao [1 ,2 ]
Wang, Lu [1 ,2 ]
Ma, Teng [1 ,2 ]
Liu, Zhongmin [1 ,2 ,3 ,4 ,5 ]
Gao, Ling [1 ,2 ,4 ,5 ]
机构
[1] Tongji Univ, Sch Med, Shanghai East Hosp, Translat Med Ctr Stem Cell Therapy, 1800 Yuntai Rd, Shanghai 200123, Peoples R China
[2] Tongji Univ, Shanghai East Hosp, Inst Regenerat Med, Sch Med, 1800 Yuntai Rd, Shanghai 200123, Peoples R China
[3] Tongji Univ, Shanghai East Hosp, Dept Cardiovasc & Thorac Surg, Sch Med, Shanghai 200120, Peoples R China
[4] Tongji Univ, Shanghai East Hosp, Shanghai Inst Stem Cell Res & Clin Translat, Shanghai 200120, Peoples R China
[5] Shanghai Engn Res Ctr Stem Cell Clin Treatment, Shanghai 200123, Peoples R China
关键词
hiPSC-ECs; Exosomes; Ca2+ homeostasis; miR-100-5p; CONTRACTILE DYSFUNCTION; CARDIAC-CELLS; REPAIR; MODEL; DIFFERENTIATION; CARDIOMYOCYTES; ANGIOGENESIS; DEGRADATION; MODULATION; MECHANISMS;
D O I
10.1186/s13287-023-03462-w
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Background Human induced pluripotent stem cell-derived endothelial cells (hiPSC-ECs) exhibit the potential to repair the injured heart after myocardial infarction (MI) by promoting neovascularization and cardiomyocyte survival. However, because of the low cellular retention and poor engraftment efficacy, cell therapy of MI is partly mediated by exosomes secreted from the transplanted cells. In this study, we investigated whether exosomes secreted from hiPSC-ECs could become a promising acellular approach to repair the infarcted heart after MI and elucidated the underlying protective mechanism.Methods The hiPSC-ECs were differentiated, and exosomes were isolated in vitro. Then, hiPSC-EC exosomes were delivered by intramyocardial injection in a murine MI model in vivo. Echocardiography, combined with hemodynamic measurement, histological examination, Ca2+ transient and cell shortening assessment, and Western blot, was used to determine the protective effects of hiPSC-EC exosomes on the infarcted heart. Furthermore, microRNA sequencing, luciferase activity assay, and microRNA gain-loss function experiments were performed to investigate the enriched microRNA and its role in exosome-mediated effects.Results In vitro, the hiPSC-EC exosomes enhanced intracellular Ca2+ transients, increased ATP content, and improved cell survival to protect cardiomyocytes from oxygen-glucose deprivation injury. Congruously, hiPSC-EC exosome administration in vivo improved the myocardial contractile function and attenuated the harmful left ventricular remodeling after MI without increasing the frequency of arrhythmias. Mechanistically, the hiPSC-EC exosomes notably rescued the protein expression and function of the sarcoplasmic reticulum Ca2+ ATPase 2a (SERCA-2a) and ryanodine receptor 2 (RyR-2) to maintain intracellular Ca2+ homeostasis and increase cardiomyocyte contraction after MI. The microRNA sequencing showed that miR-100-5p was the most abundant microRNA in exosomes. miR-100-5p could target protein phosphatase 1 beta (PP-1 beta) to enhance phospholamban (PLB) phosphorylation at Ser(16) and subsequent SERCA activity, which contributes to the hiPSC-EC exosome-exerted cytoprotective effects on maintaining intracellular Ca2+ homeostasis and promoting cardiomyocyte survival.Conclusion The hiPSC-EC exosomes maintain cardiomyocyte Ca2+ homeostasis to improve myocardial recovery after MI, which may provide an acellular therapeutic option for myocardial injury.
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页数:20
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