Discovery, characterization, and adipocyte differentiation regulation in perirenal adipose tissue of large yellow croaker (Larimichthys crocea)

被引：5

作者：

Xu, Dan ^{[1
,2
]}

Gong, Ye ^{[1
,2
]}

Xiang, Xiaojun ^{[1
,2
]}

Liu, Yongtao ^{[1
,2
]}

Mai, Kangsen ^{[1
,2
,3
]}

Ai, Qinghui ^{[1
,2
,3
]}

机构：

[1] Ocean Univ China, Key Lab Aquaculture Nutr & Feed, Minist Agr & Rural Affairs, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China

[2] Ocean Univ China, Key Lab Mariculture, Minist Educ, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China

[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, 1 Wenhai Rd, Qingdao 266237, Shandong, Peoples R China

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 2023年 / 49卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Perirenal adipose tissue; Lipid accumulation; Adipocyte differentiation; Large yellow croaker; SEA BREAM; IN-VITRO; GENE-EXPRESSION; BINDING-PROTEIN; FATTY-ACIDS; STEM-CELLS; PROLIFERATION; PREADIPOCYTES; QUANTIFICATION; ADIPOGENESIS;

D O I：

10.1007/s10695-023-01208-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adipose tissue is an essential tissue for lipid deposition in fish and is associated with excess lipid accumulation in aquaculture. However, the knowledge of the distribution and characterization of adipose tissue in fish still needs further investigation. This study for the first time discovered perirenal adipose tissue (PAT) in large yellow croaker by MRI and CT technologies. Then, the morphological and cytological characteristics of PAT were observed, showing a typical characteristic of white adipose tissue. Meanwhile, the mRNA expression of marker genes of white adipose tissue was highly expressed in PAT compared with the liver and muscle in large yellow croaker. Moreover, based on the discovery of PAT, preadipocytes from PAT were isolated, and the differentiation system of preadipocytes was established. The lipid droplet and TG content of cell were gradually increased during adipocyte differentiation. In addition, mRNA expressions of lipoprotein lipase, adipose triglyceride lipase, and transcription factors related to adipogenesis (cebp & alpha;, srebp1, ppar & alpha;, and ppar & gamma;) were quantified to explain the regulation mechanism during the differentiation process. In summary, the present study first discovered perirenal adipose tissue in fish, then explored the characterization of PAT, and revealed the regulation of adipocyte differentiation. These results could advance the understanding of adipose tissue in fish and provide a novel idea for the study of the mechanism of lipid accumulation.

引用

页码：627 / 639

页数：13