Effects of Creatine Supplementation on the Myostatin Pathway and Myosin Heavy Chain Isoforms in Different Skeletal Muscles of Resistance-Trained Rats

被引：2

作者：

de Carvalho, Marianna Rabelo ^{[1
]}

Duarte, Ellen Fernandes ^{[1
]}

Mendonca, Maria Lua Marques ^{[1
]}

de Morais, Camila Souza ^{[1
]}

Ota, Gabriel Elias ^{[1
]}

Gaspar-Junior, Jair Jose ^{[1
]}

Filiu, Wander Fernando de Oliveira ^{[2
]}

Damatto, Felipe Cesar ^{[3
]}

Okoshi, Marina Politi ^{[3
]}

Okoshi, Katashi ^{[3
]}

Oliveira, Rodrigo Juliano ^{[1
]}

Martinez, Paula Felippe ^{[1
,4
]}

de Oliveira-Junior, Silvio Assis ^{[1
,4
]}

机构：

[1] Fed Univ Mato Grosso Sul UFMS, Grad Program Hlth & Dev Midwestern Reg, BR-79070900 Campo Grande, MS, Brazil

[2] Fed Univ Mato Grosso Sul UFMS, Fac Pharmaceut Sci Food & Nutr, BR-79070900 Campo Grande, MS, Brazil

[3] Sao Paulo State Univ UNESP, Botucatu Med Sch, Internal Med Dept, BR-18618687 Botucatu, SP, Brazil

[4] Fed Univ Mato Grosso Sul UFMS, Grad Program Movement Sci, BR-79070900 Campo Grande, MS, Brazil

来源：

NUTRIENTS | 2023年 / 15卷 / 09期

关键词：

strength training; myostatin; muscle anabolism; creatine; muscle fibers; FIBER-TYPE COMPOSITION; GENE-EXPRESSION; MUSCULAR PERFORMANCE; ORAL CREATINE; EXERCISE; HYPERTROPHY; ADAPTATIONS; STRENGTH; MASS; INTENSITY;

D O I：

10.3390/nu15092224

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Creatine has been used to maximize resistance training effects on skeletal muscles, including muscle hypertrophy and fiber type changes. This study aimed to evaluate the impact of creatine supplementation on the myostatin pathway and myosin heavy chain (MyHC) isoforms in the slow- and fast-twitch muscles of resistance-trained rats. Twenty-eight male Wistar rats were divided into four groups: a sedentary control (Cc), sedentary creatine supplementation (Cr), resistance training (Tc), and resistance training combined with creatine supplementation (Tcr). Cc and Tc received standard commercial chow; Cr and Tcr received a 2% creatine-supplemented diet. Tc and Tcr performed a resistance training protocol on a ladder for 12 weeks. Morphology, MyHC isoforms, myostatin, follistatin, and ActRIIB protein expressions were analyzed in soleus and white gastrocnemius portion samples. The results were analyzed using two-way ANOVA and Tukey's test. Tc and Tcr exhibited higher performance than their control counterparts. Resistance training increased the ratio between muscle and body weight, the cross-sectional area, as well as the interstitial collagen fraction. Resistance training alone increased MyHC IIx and follistatin while reducing myostatin (p < 0.001) and ActRIIB (p = 0.040) expressions in the gastrocnemius. Resistance training induced skeletal muscle hypertrophy and interstitial remodeling, which are more evident in the gastrocnemius muscle. The effects were not impacted by creatine supplementation.

引用

页数：18

共 84 条

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