Levels of active forms of MMP-1, MMP-2, and MMP-9 as independent prognostic factors for differentiating the stage and type of lung cancer (SCLC and NSCLC)

被引:0
作者
Winkler, Karolina [1 ]
Kowalczyk, Agata [1 ]
Bereza, Paulina [1 ]
Regulska, Karolina [1 ]
Kasprzak, Artur [2 ]
Bamburowicz-Klimkowska, Magdalena [1 ,3 ]
Nowicka, Anna M. [1 ]
机构
[1] Univ Warsaw, Fac Chem, Pasteura Str 1, PL-02093 Warsaw, Poland
[2] Warsaw Univ Technol, Fac Chem, Noakowskiego Str 3, PL-00664 Warsaw, Poland
[3] Med Univ Warsaw, Fac Pharm, Banacha Str 1, PL-02097 Warsaw, Poland
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2024年 / 406卷
关键词
Metalloproteinases; Non -small cell lung cancer; Small cell lung cancer; Voltammetric detection; MMP activity; CIRCULATING TUMOR-CELLS; PHASE PEPTIDE SYNTHESIS; MATRIX METALLOPROTEINASES; POTENTIAL BIOMARKER; SERUM; EXPRESSION; INVASION; IMPACT; BLOOD; METASTASIS;
D O I
10.1016/j.snb.2024.135421
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Despite years of research on the development and treatment of cancer, its diagnosis still remains at an unsatisfactory level. Constant efforts are needed to identify new and more specific tumor markers for improving early diagnosis. Metalloproteinases (MMPs) are interesting candidates, worthy of consideration, given their potential impact on tumor progression through changes in both their levels and activity. In this study, we propose a biosensor featuring a single-step design and voltammetric detection to monitor both the levels and the activities of three MMPs, MMP-1, MMP-2, and MMP-9, particularly relevant in the context of lung cancer, during a single measurement. To simplify the biosensor construction as much as possible, receptors in the form of thiolated tripeptides, labeled with redox probes (highly specific for each MMP action), were synthesized. Each receptor was labeled with a different redox probe to enable the simultaneous detection of the three selected MMPs. The developed biosensor was characterized by its high stability, high sensitivity, and low detection limits of 10.5, 13.7, and 8.4 fg & sdot;mL- 1 for MMP-1, MMP-2, and MMP-9, respectively. To prove that MMPs are excellent biomarkers not only for the early diagnosis of lung cancer but also for distinguishing the type of cancer and determining the stage of the disease, the studies were conducted on tissue extracts obtained from mice implanted with H446 and A549 cell lines, representing small cell and non-small cell lung cancer, respectively.
引用
收藏
页数:13
相关论文
共 70 条
[1]   A model based on the quantification of complement C4c, CYFRA 21-1 and CRP exhibits high specificity for the early diagnosis of lung cancer [J].
AJONA, D. A. N. I. E. L. ;
REMIREZ, A. N. A. ;
SAINZ, C. R. I. S. T. I. N. A. ;
BERTOLO, C. R. I. S. T. I. N. A. ;
GONZALEZ, A. L. V. A. R. O. ;
VARO, N. E. R. E. A. ;
LOZANO, M. A. R. I. A. D. ;
ZULUETA, J. A. V. I. E. R. J. ;
MESA-GUZMAN, M. I. G. U. E. L. ;
MARTIN, A. N. A. C. ;
PEREZ-PALACIOS, R. O. S. A. ;
PEREZ-GRACIA, J. O. S. E. L. U. I. S. ;
MASSION, P. I. E. R. R. E. P. ;
MONTUENGA, L. U. I. S. M. ;
PIO, R. U. B. E. N. .
TRANSLATIONAL RESEARCH, 2021, 233 :77-91
[2]   A miRNA-based diagnostic model predicts resectable lung cancer in humans with high accuracy [J].
Asakura, Keisuke ;
Kadota, Tsukasa ;
Matsuzaki, Juntaro ;
Yoshida, Yukihiro ;
Yamamoto, Yusuke ;
Nakagawa, Kazuo ;
Takizawa, Satoko ;
Aoki, Yoshiaki ;
Nakamura, Eiji ;
Miura, Junichiro ;
Sakamoto, Hiromi ;
Kato, Ken ;
Watanabe, Shun-ichi ;
Ochiya, Takahiro .
COMMUNICATIONS BIOLOGY, 2020, 3 (01)
[3]   Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein [J].
Bartold, Katarzyna ;
Iskierko, Zofia ;
Borowicz, Pawel ;
Noworyta, Krzysztof ;
Lin, Chu-Yun ;
Kalecki, Jakub ;
Sharma, Piyush Sindhu ;
Lin, Hung-Yin ;
Kutner, Wlodzimierz .
BIOSENSORS & BIOELECTRONICS, 2022, 208
[4]   Integrating genomic features for non-invasive early lung cancer detection [J].
Chabon, Jacob J. ;
Hamilton, Emily G. ;
Kurtz, David M. ;
Esfahani, Mohammad S. ;
Moding, Everett J. ;
Stehr, Henning ;
Schroers-Martin, Joseph ;
Nabet, Barzin Y. ;
Chen, Binbin ;
Chaudhuri, Aadel A. ;
Liu, Chih Long ;
Hui, Angela B. ;
Jin, Michael C. ;
Azad, Tej D. ;
Almanza, Diego ;
Jeon, Young-Jun ;
Nesselbush, Monica C. ;
Keh, Lyron Co Ting ;
Bonilla, Rene F. ;
Yoo, Christopher H. ;
Ko, Ryan B. ;
Chen, Emily L. ;
Merriott, David J. ;
Massion, Pierre P. ;
Mansfield, Aaron S. ;
Jen, Jin ;
Ren, Hong Z. ;
Lin, Steven H. ;
Costantino, Christina L. ;
Burr, Risa ;
Tibshirani, Robert ;
Gambhir, Sanjiv S. ;
Berry, Gerald J. ;
Jensen, Kristin C. ;
West, Robert B. ;
Neal, Joel W. ;
Wakelee, Heather A. ;
Loo, Billy W., Jr. ;
Kunder, Christian A. ;
Leung, Ann N. ;
Lui, Natalie S. ;
Berry, Mark F. ;
Shrager, Joseph B. ;
Nair, Viswam S. ;
Haber, Daniel A. ;
Sequist, Lecia V. ;
Alizadeh, Ash A. ;
Diehn, Maximilian .
NATURE, 2020, 580 (7802) :245-+
[5]   Upregulation of microRNA-137 expression by Slug promotes tumor invasion and metastasis of non-small cell lung cancer cells through suppression of TFAP2C [J].
Chang, Tzu-Hua ;
Tsai, Meng-Feng ;
Gow, Chien-Hung ;
Wu, Shang-Gin ;
Liu, Yi-Nan ;
Chang, Yih-Leong ;
Yu, Sung-Liang ;
Tsai, Hsing-Chen ;
Lin, Shih-Wen ;
Chen, Yen-Wei ;
Kuo, Po-Yen ;
Yang, Pan-Chyr ;
Shih, Jin-Yuan .
CANCER LETTERS, 2017, 402 :190-202
[6]   Anti-metastatic effects of isolinderalactone via the inhibition of MMP-2 and up regulation of NM23-H1 expression in human lung cancer A549 cells [J].
Chuang, Cheng-Hung ;
Wang, Li-Yu ;
Wong, Yuen Man ;
Lin, En-Shyh .
ONCOLOGY LETTERS, 2018, 15 (04) :4690-4696
[7]   Dual mode fluorometric and colorimetric detection of matrix metalloproteinase MMP-9 as a cancer biomarker based on AuNPs@gelatin/ AuNCs nanocomposite [J].
Dadmehr, Mehdi ;
Mortezaei, Monireh ;
Korouzhdehi, Behnaz .
BIOSENSORS & BIOELECTRONICS, 2023, 220
[8]   Biomarkers and Lung Cancer Early Detection: State of the Art [J].
Dama, Elisa ;
Colangelo, Tommaso ;
Fina, Emanuela ;
Cremonesi, Marco ;
Kallikourdis, Marinos ;
Veronesi, Giulia ;
Bianchi, Fabrizio .
CANCERS, 2021, 13 (15)
[9]   MMP proteolytic activity regulates cancer invasiveness by modulating integrins [J].
Das, Alakesh ;
Monteiro, Melissa ;
Barai, Amlan ;
Kumar, Sandeep ;
Sen, Shamik .
SCIENTIFIC REPORTS, 2017, 7
[10]   Mortality of lung cancer as a second primary malignancy: A population-based cohort study [J].
Deng, Lei ;
Hardardottir, Hronn ;
Song, Huan ;
Xiao, Zhengrui ;
Jiang, Changchuan ;
Wang, Qian ;
Valdimarsdottir, Unnur ;
Cheng, Haiying ;
Loo, Billy W. ;
Lu, Donghao .
CANCER MEDICINE, 2019, 8 (06) :3269-3277