Practical considerations for rapid and quantitative NMR-based metabolomics

被引：13

作者：

Mulder, Frans A. A. ^{[1
]}

Tenori, Leonardo ^{[2
,3
,4
]}

Licari, Cristina ^{[2
,3
,4
]}

Luchinat, Claudio ^{[2
,3
,4
,5
]}

机构：

[1] Johannes Kepler Univ Linz, Linz, Austria

[2] Univ Florence, Magnet Resonance Ctr CERM, Florence, Italy

[3] Univ Florence, Dept Chem Ugo Schiff, Florence, Italy

[4] Consorzio Interuniv Risonanze Magnet Met Prot CIRM, Florence, Italy

[5] GiottoBiotech Srl, Florence, Italy

来源：

JOURNAL OF MAGNETIC RESONANCE | 2023年 / 352卷

关键词：

Metabolomics; NMR data acquisition; bucketing; paramagnetic NMR; Temperature stabilization; PARAMAGNETIC RELAXATION ENHANCEMENT; MRI CONTRAST AGENTS; PHENOTYPES; DYNAMICS;

D O I：

10.1016/j.jmr.2023.107462

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR is a key technology for metabolomics because of its robustness and reproducibility. Herein we discuss practical considerations that extend the utility of NMR spectroscopy. First, the long T1 spin relaxation times of small molecules limits high-throughput data acquisition because most experimental time is lost while waiting for signal recovery. In principle, the addition of a small amount of commercially-available paramagnetic gadolinium chelate allows cost-effective and efficient highthroughput mixture analysis with correct concentration determination. However, idle time caused by slow temperature regulation during sample exchanges, poses a next constraint. We show how, with proper care, NMR sample scanning times can be reduced additionally by a factor of two. Lastly, we describe how equidistant bucketing is a simple and fast procedure for metabolomic fingerprinting. The combination of these advancements help to make NMR metabolomics more versatile than it is today. (c) 2023 Elsevier Inc. All rights reserved.

引用

页数：10

共 29 条

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