Phylogenetic analysis based on whole genome sequence of bovine leukemia virus in cattle under 3 years old with enzootic bovine leukosis

被引：6

作者：

Maezawa, Masaki ^{[1
]}

Fujii, Yuki ^{[2
]}

Akagami, Masataka ^{[2
]}

Kawakami, Junko ^{[2
]}

Inokuma, Hisashi ^{[1
,3
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Lab OSG Vet Sci Global Dis Management, Bunkyo-ku, Tokyo, Japan

[2] Ibaraki Prefecture Kenpoku Livestock Hyg Serv Ctr, Mito, Ibaraki, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Farm Anim Med, Bunkyo-ku, Tokyo, Japan

来源：

PLOS ONE | 2023年 / 18卷 / 01期

基金：

日本学术振兴会;

关键词：

T-CELL LEUKEMIA; TAX GENE; PROVIRUS; MUTATION; REGION;

D O I：

10.1371/journal.pone.0279756

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Enzootic bovine leukosis (EBL) is one of bovine neoplasms caused by bovine leukemia virus (BLV). Although EBL is typically observed in cattle over 3 years old, several cases of EBL onset in cattle under 3 years old have been reported in Japan. The mechanism for EBL onset in young cattle remains unclear. Although genetic variation of BLV is limited, the variations could affect viral properties relating to BLV pathogenesis. The purpose of this study was to clarify relationship between early onset of EBL and BLV groups. Moreover, we also aimed to characterize BLV that cause early onset of EBL. Whole genome sequences of BLV in 72 EBL cattle under 3 years old and 50 EBL cattle over 3 years old were identified. Phylogenetic analysis showed that BLV was divided into 4 groups (A, B-1, B-2 and Other). The BLV from EBL cattle under 3 years old were mainly classified as group A and B-1, while those from EBL cattle over 3 years old were mainly included in group B-2. Common sequence of group A and B-1 was compared with those of group B-2. Specific sequences in LTRs, gag-pro-pol, env and tax gene regions were identified in these groups. Amino acid substitutions of Pro and Tax protein were predicted in those nucleotide sequences. Those genetic variations might contribute to the early onset of EBL.

引用

页数：8